Case Report: mutation screening in South African patients with Marfan syndrome.

Marfan syndrome (MFS) is a systemic heritable connective tissue disorder caused by pathogenic variants in the gene. Previous studies have documented the clinical utility of mutation screening as some nucleotide changes and functional domains are associated with specific clinical presentations, many of which are age dependent. However, molecular testing has not been incorporated into routine clinical service for MFS in South Africa. Here we present clinical phenotypes and molecular confirmation of MFS in a cohort of South African patients. Mutation screening using a targeted next-generation sequencing (NGS) panel identified seven heterozygous likely pathogenic and/or pathogenic variants in eleven South African patients with MFS. Two of these variants are novel. This study thus contributes to the description of the mutation spectrum of MFS in Africa and highlights the diagnostic utility and importance of -based mutation testing, especially in children and for prognostic purposes.