视黄酸、视黄酸结合蛋白和视黄醇棕榈酸酯水解酶在不同类型大鼠肝细胞中的分布。

Retinoids, retinoid-binding proteins, and retinyl palmitate hydrolase distributions in different types of rat liver cells.

作者信息

Blaner W S, Hendriks H F, Brouwer A, de Leeuw A M, Knook D L, Goodman D S

出版信息

J Lipid Res. 1985 Oct;26(10):1241-51.

Abstract

A study was conducted to determine the levels and distributions of retinoids, retinol-binding protein (RBP), retinyl palmitate hydrolase (RPH), cellular retinol-binding protein (CRBP), and cellular retinoic acid-binding protein (CRABP) in different types of isolated liver cells. Highly purified fractions of parenchymal, fat-storing (stellate), endothelial, and Kupffer cells were isolated in high yield from rat livers. The retinoid content of each fraction was measured by HPLC analysis. RBP, CRBP, and CRABP were measured by sensitive and specific radioimmunoassays, and RPH activity was measured by a sensitive microassay. The concentrations of each parameter expressed per 10(6) parenchymal or fat-storing cells were, respectively: retinoids, 1.5 and 83.9 micrograms of retinol equivalents; RBP, 138 and 7.4 ng; RPH, 826 and 1152 pmol FFA formed hr-1; CRBP, 470 and 236 ng; and CRABP, 5.6 and 8.7 ng. When these data were expressed on the basis of per unit mass of cellular protein, the concentrations of RPH, CRBP, and CRABP in the fat-storing cells, which contain 10-fold less protein than the large parenchymal cells, were seen to be greatly enriched over parenchymal cells. The parenchymal cells contained approximately 9% of the total retinoids, 98% of the total RBP, 90% of the total RPH activity, 91% of the total CRBP, and 71% of the total CRABP found in the liver. The fat-storing cells accounted for approximately 88% of the total retinoids, 0.7% of the total RBP, 10% of the RPH activity, 8% of the total CRBP, and 21% of the CRABP in the liver. The endothelial and Kupffer cell fractions contained very low levels of all of these parameters. Thus, the large and abundant parenchymal cells account for greater than 70% of the liver's RBP, RPH, CRBP, and CRABP; but the much smaller and less abundant fat-storing cells contain the majority of hepatic retinoids and greatly enriched concentrations of RPH, CRBP, and CRABP.

摘要

开展了一项研究，以确定不同类型的分离肝细胞中类视黄醇、视黄醇结合蛋白（RBP）、棕榈酸视黄酯水解酶（RPH）、细胞视黄醇结合蛋白（CRBP）和细胞视黄酸结合蛋白（CRABP）的水平及分布情况。从大鼠肝脏中高产率地分离出了高纯度的实质细胞、贮脂（星状）细胞、内皮细胞和库普弗细胞组分。通过高效液相色谱分析测定了每个组分中的类视黄醇含量。通过灵敏且特异的放射免疫分析法测定RBP、CRBP和CRABP，通过灵敏的微量分析法测定RPH活性。以每10⁶个实质细胞或贮脂细胞表示的各参数浓度分别为：类视黄醇，1.5和83.9微克视黄醇当量；RBP，138和7.4纳克；RPH，826和1152皮摩尔游离脂肪酸每小时形成量；CRBP，470和236纳克；以及CRABP，5.6和8.7纳克。当这些数据以每单位质量细胞蛋白为基础表示时，发现贮脂细胞中RPH、CRBP和CRABP的浓度比大的实质细胞高很多，贮脂细胞中的蛋白含量比大的实质细胞少10倍。实质细胞含有肝脏中约9%的总类视黄醇、98%的总RBP、90%的总RPH活性、91%的总CRBP以及71%的总CRABP。贮脂细胞占肝脏中总类视黄醇的约88%、总RBP的0.7%、RPH活性的10%、总CRBP的8%以及CRABP的21%。内皮细胞和库普弗细胞组分中所有这些参数的水平都非常低。因此，数量众多的大实质细胞占肝脏RBP、RPH、CRBP和CRABP的70%以上；但数量少得多且含量较少的贮脂细胞含有肝脏中大部分的类视黄醇以及浓度大大升高的RPH、CRBP和CRABP。