[人体尿液中催产素的放射免疫测定]
[Radioimmunoassay of urinary oxytocin in man].
作者信息
Zébidi A, Geelen G, Allevard A M, Sempore B, Jarsaillon E, Meunier C, Gharib C
出版信息
C R Seances Soc Biol Fil. 1978;172(6):1155-61.
A radioimmunoassay (RIA) for oxytocin (OT) in urine is described. 125I-OT was prepared, and antibodies were raised in rabbits against OT coupled to bovine serumalbumine. This allowed us to set up of RIA for OT which limit of detection is 1.25 pg/tube (0.6 microU). The use of an extraction procedure using CG50 Amberlite is essential. The recovery after extraction reaches 70.5%. pH 5 is the optimum pH were urine samples must be stored. The superposition of the elution peak of endogenous OT on that of exogenous hormone is an argument in favour of the validity of such an extraction procedure. Daily urinary excretion of OT reaches 9.58 mU +/- 3.48 in 18 healthy young men.
本文描述了一种用于检测尿液中催产素（OT）的放射免疫分析（RIA）方法。制备了125I-OT，并在兔体内针对与牛血清白蛋白偶联的OT产生抗体。这使我们能够建立OT的RIA，其检测限为1.25 pg/管（0.6微单位）。使用CG50 Amberlite进行萃取程序至关重要。萃取后的回收率达到70.5%。pH 5是尿液样本必须储存的最佳pH值。内源性OT的洗脱峰与外源性激素的洗脱峰重叠，这支持了这种萃取程序的有效性。18名健康年轻男性的OT每日尿排泄量达到9.58微单位±3.48。
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