Alnemari Reem M, Almutairi Farooq M, Aldhafeeri Muneef M, Abduljabbar Maram H, Alzhrani Rami M, Althobaiti Yusuf S, Althobaiti Musaad M, Ramzy Sherif, Almalki Atiah H
Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taif University, P.O. Box 11099, 21944, Taif, Saudi Arabia.
Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, University of Hafr AlBatin, 39524, Hafr AlBatin, Saudi Arabia.
Sci Rep. 2025 Jul 17;15(1):25939. doi: 10.1038/s41598-025-01644-5.
Sodium oxybate is a newly approved oral treatment for narcolepsy-related cataplexy and excessive daytime sleepiness. It has been misused recreationally and implicated in drug-facilitated crimes. In this study, a highly sensitive and environmentally benign quenching spectrofluorimetric method was established for the quantification of sodium oxybate in both pharmaceutical and spiked plasma matrices. Carbon quantum dots were used as a sustainable fluorescent probe to quantify sodium oxybate via fluorescence quenching. The surface of the fluorescent probe was modified with a sodium oxybate-tetraphenylborate complex to augment selectivity. Sodium oxybate interacts with chromophore sites on the fluorescent probe, leading to a concentration-dependent reduction in the fluorescence intensity of the probe recorded at 450 nm. The method was validated in accordance with the ICH guidelines and demonstrated a good linearity within a working range of 50-600 ng/mL. The method was shown to be sensitive, with detection and quantitation limits of 14.58 and 44.18 ng/mL, respectively. This method was successfully used to quantify sodium oxybate in pharmaceutical and spiked plasma samples, with no interference. Furthermore, the environmental sustainability of the proposed method was evaluated utilizing the Analytical GREEnness calculator metric, yielding a notably elevated greenness score.
羟丁酸钠是一种新获批用于治疗发作性睡病相关猝倒和日间过度嗜睡的口服药物。它已被用于娱乐性滥用，并与药物辅助犯罪有关。在本研究中，建立了一种高灵敏度且环境友好的猝灭荧光光谱法，用于定量测定药物和加标血浆基质中的羟丁酸钠。碳量子点被用作可持续荧光探针，通过荧光猝灭来定量羟丁酸钠。荧光探针的表面用羟丁酸钠 - 四苯基硼酸盐络合物进行修饰，以提高选择性。羟丁酸钠与荧光探针上的发色团位点相互作用，导致在450nm处记录的探针荧光强度呈浓度依赖性降低。该方法按照国际人用药品注册技术协调会（ICH）指南进行了验证，在50 - 600 ng/mL的工作范围内表现出良好的线性。该方法显示出灵敏性，检测限和定量限分别为14.58和44.18 ng/mL。该方法成功用于定量测定药物和加标血浆样品中的羟丁酸钠，无干扰。此外，利用分析绿色度计算器指标评估了所提出方法的环境可持续性，得出了显著提高的绿色度分数。
