厚朴酚通过抗氧化、抗炎、神经营养和抗凋亡机制改善利血平诱导的纤维肌痛。

Honokiol ameliorates reserpine-induced fibromyalgia through antioxidant, anti-inflammatory, neurotrophic, and anti-apoptotic mechanisms.

作者信息

Khodir Suzan A, Sweed Eman M, El-Haroun Hala, Abd-Elhamid Tarek H, El Derbaly Sara A, Mahmoud Amany R, Motawea Shaimaa M

机构信息

Medical Physiology Department, Faculty of Medicine, Menoufia University, Menoufia, 32511, Egypt.

Medical Physiology Department, Menoufia National University, Menoufia, Egypt.

出版信息

Sci Rep. 2025 Jul 17;15(1):25983. doi: 10.1038/s41598-025-07209-w.

DOI:10.1038/s41598-025-07209-w

PMID:40676048

Abstract

Fibromyalgia (FM) is a chronic condition characterized by widespread musculoskeletal pain, fatigue, psychological disturbances, and sleep issues. Honokiol (HNK) is a bioactive compound known for its medicinal properties. This study evaluated HNK's effectiveness in alleviating pain, depression, and anxiety in a reserpine-induced FM rat model. Thirty male rats were divided into three groups: control, RES (FM-induced), and RES + HNK. HNK was supplemented to RES + HNK in a dose of 8 mg/kg for 21 days. Behavioral assessments included the open field, elevated plus maze, and forced swim tests, while pain was evaluated using treadmill endurance, tail flick latency, paintbrush, and rotarod tests. Brain homogenates were analyzed for neurotransmitters, antioxidants, pro-inflammatory cytokines, and gene expressions. Histopathological evaluation of spinal cords assessed markers of inflammation and apoptosis. Results showed that HNK administration improved behavior and reduced pain. This was linked to reduced levels of malondialdehyde, tumor necrosis factor-α, and prostaglandin E2, alongside increased superoxide dismutase and interleukin-10. Additionally, HNK downregulated the expression of calcitonin gene-related peptide and the JAK/STAT3 gene. These findings suggest that HNK alleviates FM symptoms through its antioxidant, anti-inflammatory, neuroprotective, and anti-apoptotic properties, indicating its potential as a therapeutic agent for FM.

摘要

纤维肌痛（FM）是一种慢性疾病，其特征为广泛的肌肉骨骼疼痛、疲劳、心理障碍和睡眠问题。厚朴酚（HNK）是一种具有药用特性的生物活性化合物。本研究评估了HNK在利血平诱导的FM大鼠模型中减轻疼痛、抑郁和焦虑的有效性。将30只雄性大鼠分为三组：对照组、RES（FM诱导组）和RES + HNK组。以8 mg/kg的剂量给RES + HNK组补充HNK，持续21天。行为评估包括旷场试验、高架十字迷宫试验和强迫游泳试验，同时使用跑步机耐力试验、甩尾潜伏期试验、毛刷试验和转棒试验评估疼痛。对脑匀浆进行神经递质、抗氧化剂、促炎细胞因子和基因表达分析。对脊髓进行组织病理学评估，以评估炎症和凋亡标志物。结果表明，给予HNK可改善行为并减轻疼痛。这与丙二醛、肿瘤坏死因子-α和前列腺素E2水平降低以及超氧化物歧化酶和白细胞介素-10增加有关。此外，HNK下调了降钙素基因相关肽和JAK/STAT3基因的表达。这些发现表明，HNK通过其抗氧化、抗炎、神经保护和抗凋亡特性减轻FM症状，表明其作为FM治疗剂的潜力。