Lee Haekyung, Han Jae-A, Ahn Hee-Sung, Kwon Soon Hyo, Kim Kyunggon, Ryu Seongho
Division of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea.
Hyonam Kidney Laboratory, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea.
Int J Obes (Lond). 2025 Jul 17. doi: 10.1038/s41366-025-01837-y.
Extracellular vesicles (EVs) are implicated in the pathophysiology of obesity and diabetes. This study aimed to analyze the impact of diabetes on EV protein dynamics in patients with obesity before and after bariatric surgery.
This prospective study assessed the serum EV proteins in 30 patients with obesity, with and without diabetes, and 37 healthy controls using liquid chromatography-tandem mass spectrometry. EV proteins were also analyzed 6 months after bariatric surgery in patients with obesity.
We found 19 differentially expressed proteins (DEPs) in EVs between healthy controls and patients with obesity. In addition, 20 DEPs between patients with obesity who had and did not have diabetes were identified. After bariatric surgery, 14 DEPs mainly involved in the immune system were identified in patients with obesity and diabetes; adiponectin (ADIPOQ), mannose binding lectin 2 (MBL2), and hornerin (HRNR) were associated with glycemic control, body mass index, and weight loss, respectively. In patients with obesity without diabetes, 13 DEPs after bariatric surgery were predominantly involved in reactive oxygen species metabolism.
Diabetes affects the differences in EV protein profiles in patients with obesity before and after bariatric surgery. These DEPs may serve as potential biomarkers and therapeutic targets for obesity-related diabetes.
细胞外囊泡（EVs）与肥胖症和糖尿病的病理生理学有关。本研究旨在分析糖尿病对肥胖症患者在减肥手术前后EV蛋白动态变化的影响。
这项前瞻性研究使用液相色谱-串联质谱法评估了30例肥胖患者（有糖尿病和无糖尿病）以及37名健康对照者的血清EV蛋白。还对肥胖患者减肥手术后6个月的EV蛋白进行了分析。
我们发现健康对照者与肥胖患者的EVs中有19种差异表达蛋白（DEPs）。此外，还鉴定出了有糖尿病和无糖尿病的肥胖患者之间的20种DEPs。减肥手术后，在肥胖合并糖尿病患者中鉴定出14种主要参与免疫系统的DEPs；脂联素（ADIPOQ）、甘露糖结合凝集素2（MBL2）和角蛋白（HRNR）分别与血糖控制、体重指数和体重减轻有关。在无糖尿病的肥胖患者中，减肥手术后的13种DEPs主要参与活性氧代谢。
糖尿病影响肥胖症患者减肥手术前后EV蛋白谱的差异。这些DEPs可能作为肥胖相关糖尿病的潜在生物标志物和治疗靶点。
