Thi Trang Nhung Trinh, Verma Swati, Ponne Saravanaraman, Meghwanshi Gautam Kumar, Schön Thomas, Kumar Rajender
Faculty of Biomedical Sciences, Phenikaa University, Yen Nghia, Ha Dong, Hanoi 12116, Vietnam.
Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, Stockholm, Sweden.
Comput Struct Biotechnol J. 2025 Jun 30;27:2924-2932. doi: 10.1016/j.csbj.2025.06.046. eCollection 2025.
Bacteriophages are viruses that infect bacteria, which are essential for controlling bacterial diversity. Among the novel aspects, phage display-based strategies are used for epitope mapping and the development of immunotherapy. A recent classification system has been developed based on the recent sequencing methods and bioinformatic tools. The unique specificity of phages is of increasing use in biocontrol, where bacteriophages are applied to target and reduce harmful bacterial populations in agriculture, food preservation and safety, offering a sustainable alternative to chemical exposure and a plausible solution to excessive misuse of antibiotics. Phage therapy has emerged as a complement to antibiotics for difficult-to-treat infectious diseases such as multi-drug resistant bacteria where other alternatives are lacking. The ability of bacteriophages to specifically target pathogenic bacteria while sparing the normal flora makes them attractive treatment options. Among the challenges are the slow uptake of phage therapy in the clinical setting, a lack of standardisation and regulatory issues. Nevertheless, phage-based strategies are likely to become a future cornerstone for biocontrol and treatment of infectious diseases.
噬菌体是感染细菌的病毒，对于控制细菌多样性至关重要。在新出现的方面中，基于噬菌体展示的策略用于表位作图和免疫疗法的开发。基于最近的测序方法和生物信息学工具，已经开发出一种新的分类系统。噬菌体独特的特异性在生物防治中的应用越来越多，在农业、食品保鲜和安全领域，噬菌体被用于靶向和减少有害细菌种群，为化学暴露提供了一种可持续的替代方案，并为抗生素的过度滥用提供了一个合理的解决方案。噬菌体疗法已成为抗生素的补充，用于治疗诸如多重耐药菌等难以治疗的传染病，而其他替代方法则不存在。噬菌体能够特异性靶向病原菌同时不影响正常菌群，这使其成为有吸引力的治疗选择。面临的挑战包括噬菌体疗法在临床环境中的应用进展缓慢、缺乏标准化以及监管问题。尽管如此，基于噬菌体的策略很可能成为未来生物防治和传染病治疗的基石。
