Han Qiqi, Zhao Fangzheng, Wu Tong, Zhao Chunyue, Li Jiahui, Huang Shuaibing, Zhang Tingbin, Xing Jinfeng
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300131, China.
Int J Biol Macromol. 2025 Aug;320(Pt 4):146117. doi: 10.1016/j.ijbiomac.2025.146117. Epub 2025 Jul 17.
Uncontrolled bleeding of wound is one of the main causes of death, and wound often has bacterial infection and inflammation to hinder wound healing. In this study, natural polyphenol procyanidin was added to gelatin and quaternized chitosan, and a pure natural sponge (GQO) was prepared by mechanical stirring, crosslinking and freeze-drying. The macroporous structure of GQO was formed by natural soybean lecithin as blowing agent combined with mechanical stirring, and the three-dimensional network structure was crosslinked by Genipin. The use of natural compounds avoids the toxicity of traditional chemical blowing agents and crosslinkers. GQO has excellent water absorption ability (2060.1 %), suitable mechanical property (28.1 kPa), biodegradability, biocompatibility, antibacterial and antioxidant properties, and shows rapid hemostasis and accelerate wound healing performances in vivo. In the rat liver injury model, the bleeding time and blood loss of GQO were significantly lower than those in gauze group, commercial hemostatic gelatin sponge group and blank group. In the rat back full-thickness skin defect model, the wound inflammation was significantly relieved by GQO treatment, and the wound healing rate reached 98.9 % on the 14th day. Therefore, GQO has great potential for rapid hemostasis and promoting wound healing.
伤口的失控出血是主要死因之一，并且伤口常伴有细菌感染和炎症，阻碍伤口愈合。在本研究中，将天然多酚原花青素添加到明胶和季铵化壳聚糖中，通过机械搅拌、交联和冷冻干燥制备了一种纯天然海绵（GQO）。GQO的大孔结构由天然大豆卵磷脂作为发泡剂结合机械搅拌形成，三维网络结构由京尼平交联。天然化合物的使用避免了传统化学发泡剂和交联剂的毒性。GQO具有优异的吸水能力（2060.1%）、合适的力学性能（28.1 kPa）、生物可降解性、生物相容性、抗菌和抗氧化性能，并且在体内表现出快速止血和加速伤口愈合的性能。在大鼠肝损伤模型中，GQO的出血时间和失血量显著低于纱布组、商用止血明胶海绵组和空白组。在大鼠背部全层皮肤缺损模型中，GQO处理可显著减轻伤口炎症，第14天时伤口愈合率达到98.9%。因此，GQO在快速止血和促进伤口愈合方面具有巨大潜力。
