一个bHLH7-PDF2模块介导转录调控以抑制拟南芥种子油积累。

A bHLH7-PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana.

作者信息

Liu Zijin, Zhao Yu, Mao Lianying, Li Dong, Song Bo, Jin Minshan, Ma Jun, Wang Jianjun, He Shuangcheng, Wang Shixiang, Hu Huan, Guo Yuan, Yang Saiqi, Chen Mingxun

机构信息

State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, National Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis, and College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.

出版信息

Plant Commun. 2025 Sep 8;6(9):101459. doi: 10.1016/j.xplc.2025.101459. Epub 2025 Jul 18.

DOI:10.1016/j.xplc.2025.101459

PMID:40684262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12447430/

Abstract

Seed oil accumulation requires precise coordination of multiple factors, and transcription factors (TFs) play crucial roles in governing this process. However, details of how TFs and crosstalk between different TF families coordinate the transcriptional network associated with seed oil accumulation remain to be clarified. Here, we report that basic helix-loop-helix7 (bHLH7) and PROTODERMAL FACTOR2 (PDF2) TFs, both highly expressed in developing embryos, negatively control seed oil accumulation. bHLH7 and PDF2 physically interact and cooperatively inhibit the transcription of MYB DOMAIN PROTEIN 96 (MYB96), PYRUVATE DEHYDROGENASE E1 BETA1 (PDH-E1β1), PDH-E1β2, and BIOTIN CARBOXYL CARRIER PROTEIN1 (BCCP1) by directly binding to their promoters. Additional genetic evidence demonstrated that bHLH7 and PDF2 act in an additive manner to repress seed oil accumulation, and this effect is partially dependent on PDH-E1β1, which significantly promotes seed oil deposition. Collectively, our results provide insights into the mechanisms by which the bHLH7-PDF2 module mediates transcriptional regulation to control oil accumulation in A. thaliana seeds.

摘要

种子油积累需要多种因素的精确协调，而转录因子（TFs）在调控这一过程中起着关键作用。然而，TFs以及不同TF家族之间的相互作用如何协调与种子油积累相关的转录网络的细节仍有待阐明。在此，我们报道了在发育中的胚中高表达的碱性螺旋-环-螺旋7（bHLH7）和原表皮因子2（PDF2）转录因子对种子油积累起负调控作用。bHLH7和PDF2发生物理相互作用，并通过直接结合到MYB结构域蛋白96（MYB96）、丙酮酸脱氢酶E1β1（PDH-E1β1）、PDH-E1β2和生物素羧基载体蛋白1（BCCP1）的启动子上，协同抑制它们的转录。额外的遗传学证据表明，bHLH7和PDF2以累加的方式抑制种子油积累，且这种效应部分依赖于显著促进种子油沉积的PDH-E1β1。总的来说，我们的结果为bHLH7-PDF2模块介导转录调控以控制拟南芥种子油积累的机制提供了见解。