Wei Chenhuinan, Zhang Yang, Cheng Qijun, Li Rulin, Xiong Rui, Wang Shengfu
Hubei Provincial Key Laboratory of Green Materials for Light Industry, School of Materials and Chemical Engineering, Hubei University of Technology, Wuhan 430068, P.R. China.
Hubei Provincial Key Laboratory of Green Materials for Light Industry, School of Materials and Chemical Engineering, Hubei University of Technology, Wuhan 430068, P.R. China.
Colloids Surf B Biointerfaces. 2025 Nov;255:114957. doi: 10.1016/j.colsurfb.2025.114957. Epub 2025 Jul 16.
Selective detection of glucose in complex biological matrices remains a major challenge for non-enzymatic electrochemical sensors due to interference from co-existing species such as ascorbic acid (AA) and uric acid (UA). Herein, we developed an amine-functionalized CuO electrocatalyst (CuO-NH), fabricated via a facile silanization strategy using 3-aminopropyltriethoxysilane (APTES) on CuO octahedron. The introduction of surface amine group effectively suppresses the interference signals of AA and UA by 34.78 % and 46.94 %, respectively, while boosting the glucose oxidation response by 1.8-fold compared with pristine CuO. The resulting CuO-NH sensor showed a wide linear range of 0.00089-1.27 mM with a high sensitivity of 1.6001 mA cm mM, and a low detection limit of 0.89 μM, along with the excellent reproducibility and long-term stability. This customized CuO-NH material ultimately exhibited accurate measurement in actual human serum. Experimental studies and theoretical calculations reveal that the amine groups selectively inhibit the adsorption of AA and UA while accelerating the glucose oxidation kinetics, thus imitating the glucose oxidase-like function. Moreover, the universality of this APTES functionalization strategy was validated across other metal oxides, highlighting its potential to address the selectivity limitations of non-enzymatic sensors and offering new opportunities for the development of non-enzymatic biosensing platforms based on micro- and nano-structured materials.
由于存在诸如抗坏血酸（AA）和尿酸（UA）等共存物质的干扰，在复杂生物基质中选择性检测葡萄糖仍然是非酶电化学传感器面临的主要挑战。在此，我们开发了一种胺官能化的CuO电催化剂（CuO-NH），它是通过使用3-氨丙基三乙氧基硅烷（APTES）在CuO八面体上进行简便的硅烷化策略制备而成。表面胺基的引入分别有效地抑制了AA和UA的干扰信号34.78%和46.94%，同时与原始CuO相比，将葡萄糖氧化响应提高了1.8倍。所得的CuO-NH传感器显示出0.00089-1.27 mM的宽线性范围，具有1.6001 mA cm mM的高灵敏度和0.89 μM的低检测限，以及出色的重现性和长期稳定性。这种定制的CuO-NH材料最终在实际人血清中表现出准确的测量结果。实验研究和理论计算表明，胺基选择性地抑制了AA和UA的吸附，同时加速了葡萄糖氧化动力学，从而模拟了类似葡萄糖氧化酶的功能。此外，这种APTES功能化策略的通用性在其他金属氧化物中得到了验证，突出了其解决非酶传感器选择性限制的潜力，并为基于微纳米结构材料的非酶生物传感平台的开发提供了新机会。
