Wang Mengyan, Huang Xiaojing, Zhou Jinwen, Liu Yanlin, Bai Yun, Sun Yue
School of Food Science and Engineering, Ningxia University, Yinchuan 750021, Ningxia, PR China.
Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan 750021, Ningxia, PR China.
Food Chem X. 2025 Jul 2;29:102725. doi: 10.1016/j.fochx.2025.102725. eCollection 2025 Jul.
During the winemaking process, the dominance of commercial active dry yeasts (ADYs) influence the interactions among microorganisms and the profiles of wine aroma. In this study, the implantation percentage of ADYs, fungal diversity and volatile profiles during the industrial Cabernet Sauvignon wine fermentation from four wineries in Ningxia, China were investigated. Four species were identified by the culture-dependent method. A total of 38 volatile aromas were identified through headspace solid phase microextraction gas chromatography mass spectrometry. Interdelta sequence typing showed that FX10 strain used in Winery2 had a lower implantation percentage than that of IONYS-WF strain used in Winery4 during fermentation. High-throughput sequencing further elucidated differences in fungal community structures at different fermentation times between Winery2 and Winery4. Correlation analyses between microbial communities and flavor components highlighted the role of dominant species in shaping characteristic flavor profiles. This study deepens comprehension of how ADYs influence fungal succession and wine characteristics.
在葡萄酒酿造过程中,商业活性干酵母(ADY)的主导地位会影响微生物之间的相互作用以及葡萄酒的香气特征。在本研究中,对中国宁夏四个酒庄赤霞珠葡萄酒工业发酵过程中ADY的植入率、真菌多样性和挥发性成分进行了调查。通过依赖培养的方法鉴定出了四个物种。通过顶空固相微萃取气相色谱 - 质谱联用技术共鉴定出38种挥发性香气。基因间隔区序列分型显示,在发酵过程中,酒庄2使用的FX10菌株的植入率低于酒庄4使用的IONYS - WF菌株。高通量测序进一步阐明了酒庄2和酒庄4在不同发酵时间真菌群落结构的差异。微生物群落与风味成分之间的相关性分析突出了优势物种在塑造特征风味方面的作用。本研究加深了对ADY如何影响真菌演替和葡萄酒特性的理解。