Zhang Zuoyong, Dong JingJing, Zheng Li, Chen Yaopeng, Fang Chao, Chen Jiayi, Guo Jingwen, Sun Hanju, Guo Na, Fang Xue, Zhu Guilan
School of Biology and Food Engineering, Anhui Province Green Food Collaborative Technology Service Center for Rural Revitalization, Hefei Normal University, Hefei 230601, Anhui Province, PR China.
School of Food and Biological Engineering, Engineering Research Center of Bio-process of Ministry of Education, Key Laboratory for Agricultural Products Procssing of Anhui Province, Hefei University of Technology, Hefei 230009, Anhui, PR China.
Food Chem X. 2025 Jun 26;29:102695. doi: 10.1016/j.fochx.2025.102695. eCollection 2025 Jul.
This study aimed to develop a low-beany Maillard brown soy yogurt (MBSY) and compare its physicochemical properties and flavor with non-Maillard soy yogurt, commercial soy yogurt, and commercial brown yogurt. Response surface optimization determined key process parameters for MBSY: glucose addition of 7.87%, glycine addition of 1.25%, and browning at 95 °C for 2 h. The obtained MBSY showed favorable titratable acidity (74.54 ± 0.23°T) and water-holding capacity (55.67 ± 0.08%), with enhanced DPPH and ABTS free radical scavenging activities of 55.35% and 51.16% and lactic acid bacteria viability of 2.59 × 10 CFU/mL. Textural and rheological analysis showed that the Maillard reaction could reduce the hardness, cohesiveness, and viscoelasticity of MBSY due to disordered protein aggregation forming loose gel networks. -nose and GC-MS/IMS revealed that MBSY exhibited low-beany compounds and increased yogurt-like and roasted notes. This work demonstrates the potential of the Maillard reaction in improving the functionality and flavor of plant-based dairy products.
本研究旨在开发一种低豆腥味的美拉德褐变大豆酸奶(MBSY),并将其理化性质和风味与非美拉德大豆酸奶、市售大豆酸奶和市售褐变酸奶进行比较。响应面优化确定了MBSY的关键工艺参数:葡萄糖添加量为7.87%,甘氨酸添加量为1.25%,在95℃下褐变2小时。所获得的MBSY表现出良好的可滴定酸度(74.54±0.23°T)和持水能力(55.67±0.08%),DPPH和ABTS自由基清除活性增强,分别为55.35%和51.16%,乳酸菌活力为2.59×10 CFU/mL。质地和流变学分析表明,美拉德反应可降低MBSY的硬度、内聚性和粘弹性,这是由于蛋白质聚集紊乱形成了松散的凝胶网络。感官评价和GC-MS/IMS显示,MBSY呈现出低豆腥味化合物,并增加了酸奶样和烘焙风味。这项工作证明了美拉德反应在改善植物基乳制品功能和风味方面的潜力。