Teo Jeanette W P, Cheng Janet W S, Wee Jamie S, Chan Douglas, Chew Ka Lip
Department of Laboratory Medicine, National University Hospital, Singapore 119074, Singapore.
Ng Teng Fong General Hospital, Singapore 609606, Singapore.
Med Mycol. 2025 Jul 2;63(7). doi: 10.1093/mmy/myaf062.
During an 11-month study at two hospital sites, 33 isolates initially classified as the Trichophyton mentagrophytes complex were subjected to whole-genome sequencing. Of these, 24 (72.7%) were identified as T. indotineae and 9 (27.3%) as T. interdigitale, with no T. mentagrophytes detected. Among the 22 patients with available clinical data, 75% exhibited involvement of multiple anatomical sites; although only two patients reported recent travel, 63.6% were migrant workers. Symptom resolution was documented in only four patients, whereas five experienced recurrences. Nearly 80% of T. indotineae isolates displayed terbinafine resistance linked to squalene epoxidase mutations, while no phenotypic azole resistance was observed, consistent with the absence of cyp51 mutations. Single-nucleotide polymorphism (SNP) analysis of both T. indotineae (520-1531 SNPs) and T. interdigitale (1380-115 963 SNPs) revealed no phylogenetic clustering, suggesting independent introductions.
在两个医院进行的为期11个月的研究中，对最初归类为须癣毛癣菌复合群的33株分离株进行了全基因组测序。其中，24株（72.7%）被鉴定为印地毛癣菌，9株（27.3%）为指间毛癣菌，未检测到须癣毛癣菌。在有可用临床数据的22例患者中，75%表现为多个解剖部位受累；虽然只有2例患者报告近期有旅行史，但63.6%为农民工。仅4例患者有症状缓解记录，而5例出现复发。近80%的印地毛癣菌分离株显示出与角鲨烯环氧酶突变相关的特比萘芬耐药性，而未观察到表型唑类耐药性，这与未检测到cyp51突变一致。对印地毛癣菌（520 - 1531个单核苷酸多态性）和指间毛癣菌（1380 - 115963个单核苷酸多态性）的单核苷酸多态性分析均未发现系统发育聚类，表明是独立引入的。
