Huang Yong-Xin, Zhu Xin-Hua, Jia Ju-Fang, Jiang Si-Liang, Liang Jun, Kuang Hai-Xue, Xia Yong-Gang
Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China.
J Agric Food Chem. 2025 Jul 30;73(30):19047-19064. doi: 10.1021/acs.jafc.5c04211. Epub 2025 Jul 21.
Two homogalacturonan (HG)-type pectic polysaccharides, (, 5.37 × 10 g/mol) and (, 6.10 × 10 g/mol), were isolated from fruits. Their structures were determined to consist of repeating units of →1)-αd-GalA-(4→, exhibiting varying degrees of esterification based on multiple chromatographic analysis techniques. The possible distribution of methyl esters in the HG region of and was elucidated using endopolygalacturonase (endo-PG) hydrolysis combined with HILIC-ESI-QTOF-MS/MS and UPSEC-ESI-MS. Surface plasmon resonance analysis revealed that both ( = 1.12 × 10 and 2.27 × 10 M for TLR2/4) and ( = 4.80 × 10 and 6.68 × 10 M for TLR2/4) exhibited strong binding affinities to TLR2/4. However, showed approximately twice the affinity for TLR2/4 than . The in vitro assessment of immunomodulatory activity further confirmed that significantly promoted the secretion of NO and upregulated the levels of TNF-α and IL-6 more effectively than . These results indicate that may display a more significant immune activation capability due to its higher affinity for TLR2/4.
从果实中分离出两种同型半乳糖醛酸聚糖（HG）型果胶多糖，即（，5.37×10 g/mol）和（，6.10×10 g/mol）。基于多种色谱分析技术，确定它们的结构由→1)-α-d-半乳糖醛酸-(4→的重复单元组成，并表现出不同程度的酯化作用。使用内切多聚半乳糖醛酸酶（endo-PG）水解结合亲水相互作用液相色谱-电喷雾电离-四极杆飞行时间串联质谱（HILIC-ESI-QTOF-MS/MS）和超高效尺寸排阻色谱-电喷雾电离质谱（UPSEC-ESI-MS）阐明了和在HG区域中甲酯的可能分布。表面等离子体共振分析表明，（对TLR2/4的 = 1.12×10和2.27×10 M）和（对TLR2/4的 = 4.80×10和6.68×10 M）均对TLR2/4表现出强结合亲和力。然而，对TLR2/4的亲和力约为的两倍。免疫调节活性的体外评估进一步证实，比更有效地显著促进了NO的分泌并上调了TNF-α和IL-6的水平。这些结果表明，由于其对TLR2/4具有更高的亲和力，可能表现出更显著的免疫激活能力。
