Whitty Annie L, Jennings Staci K, Lyons Hannah E, Thompson Jeremy G, Mcpherson Nicole O, Kind Karen L, Dunning Kylie R, Nottle Mark B
Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia.
Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia; Freemason Centre for Male Health and Wellbeing, The University of Adelaide, South Australia, Australia; Genea, Level 2/321 Kent Street, Sydney, New South Wales, Australia.
Theriogenology. 2025 Nov;247:117589. doi: 10.1016/j.theriogenology.2025.117589. Epub 2025 Jul 16.
In vitro embryo production (IVP) is a reproductive technology used in the cattle industry to increase the rate of genetic gain. The present study determined if addition of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) during in vitro oocyte maturation (IVM) could improve in vitro embryo production in cattle. Cumulus oocyte complexes (COCs) were aspirated from abattoir derived ovaries and matured in the absence or presence (10 ng/ml) of rbGM-CSF. Following IVM, COCs were fertilized and cultured in vitro, and embryo development assessed. The effects of rbGM-CSF on mitochondrial activity, glucose uptake, reactive oxygen species levels, and metabolic gene expression in COCs were examined. Compared with the control, addition of rbGM-CSF during IVM increased blastocyst development rate (+12 %; p < 0.01 and hatching blastocyst rate (+24 %; p < 0.05). Inclusion of rbGM-CSF also increased the number of cells within the inner cell mass (+27 %; p < 0.05), and the ratio of inner cell mass to trophectoderm cells (+14 %; p < 0.05) in resultant blastocyst-stage embryos. The total blastocyst cell number also tended to be higher in the rbGM-CSF group (+7 %; p < 0.1). Addition of rbGM-CSF increased mitochondrial activity in oocytes (+10 %; p < 0.05), and glucose uptake in both cumulus cells (+316 %; p < 0.05) and oocytes (+190 %; p < 0.05). There was no effect of rbGM-CSF on COC gene expression of metabolic related genes. In conclusion, we have shown that the addition of rbGM-CSF during IVM improves bovine IVP.
体外胚胎生产（IVP）是一种用于养牛业以提高遗传增益率的繁殖技术。本研究确定了在体外卵母细胞成熟（IVM）过程中添加重组牛粒细胞-巨噬细胞集落刺激因子（rbGM-CSF）是否能提高牛的体外胚胎生产效率。从屠宰场获得的卵巢中抽吸卵丘卵母细胞复合体（COC），并在不存在或存在（10 ng/ml）rbGM-CSF的情况下进行成熟培养。IVM后，使COC受精并进行体外培养，并评估胚胎发育情况。检测了rbGM-CSF对COC中线粒体活性、葡萄糖摄取、活性氧水平和代谢基因表达的影响。与对照组相比，在IVM过程中添加rbGM-CSF可提高囊胚发育率（提高12%；p<0.01）和孵化囊胚率（提高24%；p<0.05）。添加rbGM-CSF还增加了所得囊胚期胚胎内细胞团中的细胞数量（增加27%；p<0.05），以及内细胞团与滋养外胚层细胞的比例（增加14%；p<0.05）。rbGM-CSF组的囊胚总细胞数也有升高的趋势（增加7%；p<0.1）。添加rbGM-CSF可提高卵母细胞中的线粒体活性（提高10%；p<0.05），并增加卵丘细胞（提高316%；p<0.05）和卵母细胞（提高190%；p<0.05）中的葡萄糖摄取。rbGM-CSF对COC中代谢相关基因的基因表达没有影响。总之，我们已经证明在IVM过程中添加rbGM-CSF可提高牛的IVP效率。
