Liu Yuxuan, Zhang Sinan, Zhang Guohui, Zhang Yue, Yang Chen, Wang Zejian, Yu Junxiong, Wu Shiyong
Department of Chemical Engineering for Energy Resources, National Key Laboratory of Coal Liquification, Gasification and Utilization with High Efficiency and Low Carbon Technology, East China University of Science and Technology, Shanghai, China.
Department of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Bioresour Technol. 2025 Nov;436:133016. doi: 10.1016/j.biortech.2025.133016. Epub 2025 Jul 19.
Penicillin fermentation fungal residue (PFFR), a hazardous waste with over 90 % organic matter, can pollute the environment if not properly disposed of. In this study, acid/alkaline heat pretreatment was employed to remove antibiotic residues and break down macromolecular compounds into smaller molecules. The hydrolysate contained 17.23 g/L of total amino acids, 5.34 g/L of reducing sugars, and 33.6 g/L of salts. PFFR hydrolysate was then used as the base medium for the halophile Halomonas elongata to produce the high-value compound ectoine. Response surface methodology was used to optimize medium conditions, resulting in a maximum ectoine concentration of 3957.06 ± 214.12 mg/L. Additionally, a cost-effective mixture of corn steep liquor and hydrolysate was employed for fed-batch cultivation, combined with gradient salt shock to optimize fermentation. The final ectoine yield reached 15837.02 ± 422.27 mg/L. This study offers a cost-effective, environmentally friendly, and efficient strategy for the resource utilization of PFFR.
青霉素发酵真菌残渣（PFFR）是一种含有90%以上有机物的危险废物，若处置不当会污染环境。本研究采用酸/碱热预处理去除抗生素残留，并将大分子化合物分解为小分子。水解产物含有17.23 g/L的总氨基酸、5.34 g/L的还原糖和33.6 g/L的盐。然后将PFFR水解产物用作嗜盐菌嗜盐栖热孢菌生产高价值化合物四氢嘧啶的基础培养基。采用响应面法优化培养基条件，得到的四氢嘧啶最大浓度为3957.06±214.12 mg/L。此外，采用经济有效的玉米浆和水解产物混合物进行分批补料培养，并结合梯度盐冲击优化发酵。最终四氢嘧啶产量达到15837.02±422.27 mg/L。本研究为PFFR的资源利用提供了一种经济有效、环境友好且高效的策略。
