Kazama Itsuro, Sonobe Hiroyuki, Shida Junko
Miyagi University, School of Nursing, 1-1 Gakuen, Taiwa-cho, Kurokawa-gun, Miyagi, Japan.
Yamagata University, School of Nursing, Yamagata-shi, Yamagata, Japan.
Cell Physiol Biochem. 2025 Jul 15;59(4):465-477. doi: 10.33594/000000793.
BACKGROUND/AIMS: Magnesium and zinc are vital trace elements found in numerous foods and dietary supplements. In addition to their antioxidant, anticancer, antibacterial, and anti-inflammatory effects, clinical research has suggested that they possess anti-allergic properties.
Using differential-interference contrast (DIC) microscopy, we examined the effects of magnesium chloride (MgCl2) and zinc chloride (ZnCl2) on rat peritoneal mast cell degranulation. We also examined their effects in conjunction with adrenaline, the first-choice drug for anaphylaxis treatment.
Both MgCl2 and ZnCl2 reduced the number of degranulating mast cells in a dose-dependent manner. MgCl2 significantly decreased the number of degranulating mast cells at concentrations of 50 mM or higher, whereas ZnCl2 achieved similar effects at much lower concentrations of 25 µM or more. These levels of MgCl2 or ZnCl2 enhanced the inhibitory effects of 1 mM adrenaline on mast cell degranulation. Additionally, pharmacological inhibition of the transient receptor potential cation channel subfamily M member 7 (TRPM7) by NS8593 reduced the number of degranulating mast cells in a dose-dependent manner.
This study is the first to provide in vitro evidence that magnesium and zinc stabilize mast cells in a dose-dependent manner and also enhance the effects of adrenaline. TRPM7, which has higher permeability to zinc ions than to magnesium ions, may contribute to the stronger mast cell-stabilizing properties of zinc.
背景/目的：镁和锌是存在于多种食物及膳食补充剂中的重要微量元素。除了具有抗氧化、抗癌、抗菌及抗炎作用外，临床研究表明它们还具有抗过敏特性。
我们使用微分干涉差（DIC）显微镜检查了氯化镁（MgCl₂）和氯化锌（ZnCl₂）对大鼠腹膜肥大细胞脱颗粒的影响。我们还研究了它们与肾上腺素（过敏反应治疗的首选药物）联合使用时的效果。
MgCl₂和ZnCl₂均以剂量依赖的方式减少了脱颗粒肥大细胞的数量。MgCl₂在浓度为50 mM或更高时显著减少了脱颗粒肥大细胞的数量，而ZnCl₂在低得多的浓度（25 µM或更高）时就能达到类似效果。这些MgCl₂或ZnCl₂水平增强了1 mM肾上腺素对肥大细胞脱颗粒的抑制作用。此外，NS8593对瞬时受体电位阳离子通道亚家族M成员7（TRPM7）的药理学抑制以剂量依赖的方式减少了脱颗粒肥大细胞的数量。
本研究首次提供了体外证据，表明镁和锌以剂量依赖的方式稳定肥大细胞，并增强肾上腺素的作用。TRPM7对锌离子的通透性高于对镁离子的通透性，这可能是锌具有更强肥大细胞稳定特性的原因。
