Monaco Edoardo, Annibaldi Alessandro, Saithna Adnan, Marzilli Fabio, Carrozzo Alessandro, Visco Vincenzo, Ferretti Andrea, Ranieri Danilo
Department of Orthopaedics Sant'Andrea Hospital, La Sapienza University of Rome Rome Italy.
AZBSC Orthopedics Scottsdale Arizona USA.
J Exp Orthop. 2025 Jul 21;12(3):e70364. doi: 10.1002/jeo2.70364. eCollection 2025 Jul.
The aim of this study was to compare gene expression and cell biology in biopsy specimens from repaired and reconstructed anterior cruciate ligaments.
A biopsy of the repaired and reconstructed anterior cruciate ligaments was performed. Gene expression analysis of alpha smooth muscle alpha actin via immunofluorescence and gene expression analysis of Collagen I, Collagen III, Collagen I-III ratio, and p16 via reverse transcription polymerase chain reaction was performed. In addition, cell motility and migration were quantitatively assessed. Data analysis was performed using analysis of variance (ANOVA) and Tukey's multiple comparison tests.
Twenty-five biopsies, 15 from repaired anterior cruciate ligaments and 10 from reconstructed anterior cruciate ligaments were obtained. Gene expression analysis showed a higher expression of Collagen I in the reconstruction group ( = 0.0027) while the expression of alpha smooth muscle actine, was significantly greater for the repair group ( = 0.001). Quantitative analysis demonstrated significantly higher cell motility in the cells from repaired ligament compared to cells cultured from grafts (43 vs. 54% residual open area from scratch, = 0.0001 at 24 h; 10 vs. 17% residual open area from scratch, = 0.001 at 48 h).
Molecular analysis of repaired and reconstructed anterior cruciate ligaments demonstrates significant differences with respect to collagen quality, α-SMA, and p16. dPCs cultured from biopsies tissue showed a greater cellular activation and migration capacity in the repaired ligament. The clinical importance of these findings is a confirmation that repaired anterior cruciate ligament tissue exhibits a cellular behaviour consistent with excellent biological healing potential.
Level III, case-control study.
本研究旨在比较修复和重建的前交叉韧带活检标本中的基因表达和细胞生物学特性。
对修复和重建的前交叉韧带进行活检。通过免疫荧光对α平滑肌肌动蛋白进行基因表达分析，并通过逆转录聚合酶链反应对I型胶原、III型胶原、I-III型胶原比例和p16进行基因表达分析。此外，对细胞运动性和迁移进行定量评估。使用方差分析（ANOVA）和Tukey多重比较检验进行数据分析。
共获得25份活检标本，其中15份来自修复的前交叉韧带，10份来自重建的前交叉韧带。基因表达分析显示，重建组中I型胶原的表达较高（P = 0.0027），而修复组中α平滑肌肌动蛋白的表达明显更高（P = 0.001）。定量分析表明，与移植培养的细胞相比，修复韧带来源的细胞运动性明显更高（划痕后24小时剩余开放面积分别为43%和54%，P = 0.0001；划痕后48小时剩余开放面积分别为10%和17%，P = 0.001）。
修复和重建的前交叉韧带的分子分析表明，在胶原质量、α-SMA和p16方面存在显著差异。从活检组织培养的dPCs在修复韧带中表现出更大的细胞活化和迁移能力。这些发现的临床意义在于证实修复的前交叉韧带组织表现出与优异生物愈合潜力一致的细胞行为。
III级，病例对照研究。
