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前交叉韧带近端和远端的韧带旁组织差异能否解释其不同的愈合能力？基于韧带旁组织理论的CD34和α-SMA表达的定量及免疫组织化学分析

Do the Differences in the Epiligament of the Proximal and Distal Parts of the Anterior Cruciate Ligament Explain Their Different Healing Capacities? Quantitative and Immunohistochemical Analysis of CD34 and α-SMA Expression in Relation to the Epiligament Theory.

作者信息

Georgiev Georgi P, Yordanov Yordan, Olewnik Łukasz, Tubbs Richard Shane, LaPrade Robert F, Ananiev Julian, Slavchev Svetoslav A, Dimitrova Iva N, Gaydarski Lyubomir, Landzhov Boycho

机构信息

Department of Orthopedics and Traumatology, University Hospital Queen Giovanna-ISUL, Medical University of Sofia, 1527 Sofia, Bulgaria.

Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria.

出版信息

Biomedicines. 2024 Jan 11;12(1):156. doi: 10.3390/biomedicines12010156.

DOI:10.3390/biomedicines12010156
PMID:38255261
原文链接:
https://pmc.ncbi.nlm.nih.gov/articles/PMC10813037/
Abstract

The aim of this study was to assess the epiligament theory by determining the normal epiligament morphology of the proximal and distal parts of the anterior cruciate ligament in humans and analyzing the differences between them and the midportion of the ligament in terms of cell numbers and expression of CD34 and α-SMA. Samples were obtained from the anterior cruciate ligaments of 12 fresh knee joints. Monoclonal antibodies against CD34 and α-SMA were used for immunohistochemistry. Photomicrographs were analyzed using ImageJ software, version 1.53f. The cell density was higher in the epiligament than in the ligament connective tissue. Cell counts were higher in the proximal and distal thirds than in the midsubstance of the epiligament. CD34 was expressed similarly in the proximal and distal thirds, although it seemed slightly more pronounced in the distal third. α-SMA expression was more robust in the proximal than the distal part. The results revealed that CD34 and α-SMA are expressed in the human epiligament. The differences between the numbers of cells in the proximal and distal parts of the epiligament and the expression of CD34 and α-SMA enhance epiligament theory. Future investigations into improving the quality of ligament healing should not overlook the epiligament theory.

摘要

本研究的目的是通过确定人类前交叉韧带近端和远端的正常韧带表面形态，并分析它们与韧带中部在细胞数量以及CD34和α -平滑肌肌动蛋白（α - SMA）表达方面的差异，来评估韧带表面理论。样本取自12个新鲜膝关节的前交叉韧带。使用抗CD34和α - SMA的单克隆抗体进行免疫组织化学。使用ImageJ软件1.53f版本分析显微照片。韧带表面的细胞密度高于韧带结缔组织。韧带表面近端和远端三分之一处的细胞计数高于中部。CD34在近端和远端三分之一处的表达相似，尽管在远端三分之一处似乎稍明显一些。α - SMA在近端的表达比远端更强烈。结果表明，CD34和α - SMA在人类韧带表面表达。韧带表面近端和远端部分的细胞数量以及CD34和α - SMA表达的差异强化了韧带表面理论。未来关于提高韧带愈合质量的研究不应忽视韧带表面理论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/832972a67f44/biomedicines-12-00156-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/4b80d440c96a/biomedicines-12-00156-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/8606958ac9e9/biomedicines-12-00156-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/d51256928ad3/biomedicines-12-00156-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/6f9b6e2ab94d/biomedicines-12-00156-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/61b457356c4b/biomedicines-12-00156-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/832972a67f44/biomedicines-12-00156-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/4b80d440c96a/biomedicines-12-00156-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/8606958ac9e9/biomedicines-12-00156-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/d51256928ad3/biomedicines-12-00156-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/6f9b6e2ab94d/biomedicines-12-00156-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/61b457356c4b/biomedicines-12-00156-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ea/10813037/832972a67f44/biomedicines-12-00156-g006.jpg

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Standardized statement for the ethical use of human cadaveric tissues in anatomy research papers: Recommendations from Anatomical Journal Editors-in-Chief.关于在解剖学研究论文中使用人尸体组织的伦理规范标准声明：《解剖学杂志》总编辑的建议。
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