Nugroho Wahyunanto Agung, Izza Ni'matul, Hj Hassan Ummul Hasanah, Alsaigh Ahmad A, Wibisono Yusuf
Bioprocess Engineering, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, East Java, Indonesia.
Petroleum and Chemical Engineering Programme Area, Universiti Teknologi Brunei, Darussalam, Brunei.
Bioengineered. 2025 Dec;16(1):2531667. doi: 10.1080/21655979.2025.2531667. Epub 2025 Jul 22.
Anaerobic digestion (AD) is a sustainable technology that converts organic waste into renewable energy while reducing greenhouse gas emissions. Recent studies suggest that adding CO₂ to the AD process can improve methane production through different mechanisms. This review examines four key ways CO₂ supplementation can enhance methane yield: (1) direct conversion of CO₂ into acetate by homoacetogens, (2) direct methanation of CO₂ by hydrogenotrophic methanogens, (3) improved breakdown of organic material due to higher enzyme activity, and (4) better digester conditions through pH regulation and reduced ammonia toxicity. By analyzing microbial interactions and process improvements, this paper highlights knowledge gaps and the need for further research to optimize CO₂ addition in different operational settings. These findings are expected to contribute to the development of cost-effective and efficient AD systems that support energy recovery and environmental sustainability.
厌氧消化（AD）是一种可持续技术，可将有机废物转化为可再生能源，同时减少温室气体排放。最近的研究表明，在厌氧消化过程中添加二氧化碳可以通过不同机制提高甲烷产量。本综述探讨了补充二氧化碳可提高甲烷产量的四种关键方式：（1）同型产乙酸菌将二氧化碳直接转化为乙酸盐；（2）氢营养型产甲烷菌将二氧化碳直接甲烷化；（3）由于酶活性提高而改善有机物质的分解；（4）通过调节pH值和降低氨毒性改善消化条件。通过分析微生物相互作用和工艺改进，本文突出了知识空白以及在不同操作环境下优化二氧化碳添加量的进一步研究需求。这些发现有望促进开发具有成本效益和高效的厌氧消化系统，以支持能源回收和环境可持续性。
