Cao Mingyue, Zhu Xiaoming, Liu Yunting, Su Yangang, Hu Xinru, Liu Zhiqiang, Yu Xiaoqiang
State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.
Anal Chem. 2025 Aug 5;97(30):16547-16556. doi: 10.1021/acs.analchem.5c02739. Epub 2025 Jul 22.
Interactions between lipid droplets (LDs) and mitochondria are vital for maintaining the cellular metabolism and energy homeostasis. However, visualizing these interaction dynamics at a high resolution in live cells is still very challenging. Here, a boron-bridged near-infrared probe () was developed specifically for low-power stimulated emission depletion (STED) super-resolution imaging of LDs. exhibited excellent photostability and enabled continuous scanning of 1000+ frames using a STED laser (0.96 MW cm, 775 nm), without significant fluorescence decay. Furthermore, dynamic dual-color STED super-resolution visualization of the morphological interactions between LDs and mitochondria was achieved with the aid of commercial mitochondrial dyes. For the first time, the dynamic process of mitochondrial wrapping around the LDs and LD-mediated mitochondria tubulation were successfully captured. This study sheds light on developing ultraphotostable organic fluorescent probes for versatile low-power STED nanoscopy.
脂滴（LDs）与线粒体之间的相互作用对于维持细胞代谢和能量稳态至关重要。然而，在活细胞中以高分辨率可视化这些相互作用动态仍然非常具有挑战性。在此，专门开发了一种硼桥连近红外探针（）用于脂滴的低功率受激发射损耗（STED）超分辨率成像。该探针表现出优异的光稳定性，使用STED激光（0.96 MW/cm²，775 nm）能够连续扫描1000多个帧，且荧光无明显衰减。此外，借助商业线粒体染料实现了脂滴与线粒体之间形态相互作用的动态双色STED超分辨率可视化。首次成功捕捉到线粒体围绕脂滴的动态过程以及脂滴介导的线粒体成管过程。这项研究为开发用于通用低功率STED纳米显微镜的超光稳定有机荧光探针提供了启示。
