Tran Thanh Truc, Bui Phuc Hoang
Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), VNU-HCM, Ho Chi Minh City, Vietnam.
Faculty of Applied Technology, School of Technology, Van Lang University, Ho Chi Minh City, Vietnam.
Int J Microbiol. 2025 Jul 15;2025:7578951. doi: 10.1155/ijm/7578951. eCollection 2025.
, an opportunistic pathogen, is responsible for a wide range of healthcare-associated infections (HAIs), particularly in patients in intensive care units (ICUs). Carbapenem-resistant (CRAB) is of particular concern due to its extensive multidrug resistance (MDR) and limited treatment options. In Vietnam, CRAB has become increasingly prevalent, with resistant mechanisms primarily attributed to the presence of and genes. This study investigates the clinical characteristics and genomic epidemiology of three CRAB isolates (ICU773, ICU400, and ICU399) from a tertiary-care hospital in Ho Chi Minh City. The isolates exhibited high resistance to a wide range of antibiotics, including carbapenems, aminoglycosides, and fluoroquinolones, while maintaining susceptibility to colistin. Whole-genome sequencing was used to analyze the genomic profiles, resistance genes, and sequence types of the isolates. All three isolates possessed and additional resistance genes such as and aminoglycoside-modifying enzymes (AMEs). MLST analysis revealed distinct genetic lineages, with ICU773 and ICU400 belonging to Sequence Types 2/195 and 2/Novel and ICU399 to Sequence Types 571/804. In silico analysis further identified several efflux pump genes and other resistance mechanisms, including the presence of the , and pumps. These findings highlight the complexity of CRAB's genomic diversity and resistance mechanisms in the region, underscoring the urgent need for continuous surveillance and novel therapeutic strategies to combat this growing healthcare threat.
[病原体名称]是一种机会致病菌，可导致多种医疗保健相关感染（HAIs），尤其是在重症监护病房（ICU）的患者中。耐碳青霉烯类[病原体名称]（CRAB）因其广泛的多重耐药性（MDR）和有限的治疗选择而备受关注。在越南，CRAB已变得越来越普遍，其耐药机制主要归因于[相关基因名称1]和[相关基因名称2]基因的存在。本研究调查了来自胡志明市一家三级医院的三株CRAB分离株（ICU773、ICU400和ICU399）的临床特征和基因组流行病学。这些分离株对包括碳青霉烯类、氨基糖苷类和氟喹诺酮类在内的多种抗生素表现出高度耐药性，而对黏菌素保持敏感。全基因组测序用于分析分离株的基因组图谱、耐药基因和序列类型。所有三株分离株都拥有[相关基因名称1]以及其他耐药基因，如[相关基因名称3]和氨基糖苷类修饰酶（AMEs）。多位点序列分型（MLST）分析揭示了不同的遗传谱系，ICU773和ICU400属于序列类型2/195和2/新型，ICU399属于序列类型571/804。计算机分析进一步鉴定了几种外排泵基因和其他耐药机制，包括[相关泵名称1]、[相关泵名称2]和[相关泵名称3]泵的存在。这些发现突出了该地区CRAB基因组多样性和耐药机制的复杂性，强调了持续监测和新型治疗策略以应对这一日益严重的医疗保健威胁的迫切需求。
