Cinkajzlová A, Anderlová K, Hornová M, Pařízek A, Mráz M, Kršek M, Haluzík M, Šimják P
Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic, Department of Fetal Medicine, Gennet, Prague, Czech Republic.
Physiol Res. 2025 Jul 23;74(3):403-417. doi: 10.33549/physiolres.935424.
Pregnant women with type 1 diabetes mellitus (T1DM) are at higher risk of complication development in both mothers and their children. The present study aims to describe changes in circulating and umbilical cord concentrations of recently described predictors of pregnancy complications in a group of women with T1DM. Sixty-seven cases and 34 healthy pregnant controls were included in the study and circulatory levels of TGF-alpha, HB-EGF, BDNF, sFlt-4, PDGF, SCF, galectin-1, Fas ligand, CCL-20, P-selectin, IFNgammaR1, IL-10, IL-8, leptin, and insulin were assessed in 10 to 13, (V1), 18 to 21 (V2), 28 to 31 (V3) and 34 to 36 weeks of gestation (V4), and immediately after delivery (V5). BDNF, sFlt-4, HB-EGF, SCF, Fas ligand, galectin-1, IL-8, leptin, and insulin were higher in women with T1DM compared to controls during pregnancy (all p<0.05). While HB-EGF, CCL-20, and P-selectin correlate with maternal glucose control, circulatory SCF, P-selectin, galectin-1, PDGF, IFNgammaR1, sFlt-4, and TGF-alpha levels positively correlated with IL-10 levels suggesting that their expression is altered in the presence of inflammation. Leptin and insulin cord blood levels were higher in newborns of the mothers with T1DM relative to those without T1DM. Pregnancy of women with type 1 diabetes mellitus is associated with numerous changes in circulatory factors, but these changes are not reflected in the cord blood. The observed variations in trophic and inflammatory mediators may be linked to adverse pregnancy outcomes and could potentially be incorporated into predictive models for pregnancy complications in women with type 1 diabetes. Key words Type 1 diabetes mellitus " Serum " Plasma " Cord blood " Pregnancy complications.
患有1型糖尿病（T1DM）的孕妇出现母婴并发症的风险更高。本研究旨在描述一组T1DM女性中最近描述的妊娠并发症预测因子的循环和脐带血浓度变化。该研究纳入了67例病例和34例健康孕妇作为对照，并在妊娠10至13周（V1）、18至21周（V2）、28至31周（V3）和34至36周（V4）以及分娩后立即（V5）评估了TGF-α、HB-EGF、BDNF、sFlt-4、PDGF、SCF、半乳糖凝集素-1、Fas配体、CCL-20、P-选择素、IFNγR1、IL-10、IL-8、瘦素和胰岛素的循环水平。与对照组相比，T1DM女性在孕期的BDNF、sFlt-4、HB-EGF、SCF、Fas配体、半乳糖凝集素-1、IL-8、瘦素和胰岛素水平更高（均p<0.05）。虽然HB-EGF、CCL-20和P-选择素与母体血糖控制相关，但循环中的SCF、P-选择素、半乳糖凝集素-1、PDGF、IFNγR1、sFlt-4和TGF-α水平与IL-10水平呈正相关，表明它们在炎症存在时表达发生改变。与未患T1DM的母亲的新生儿相比，患T1DM母亲的新生儿脐带血中的瘦素和胰岛素水平更高。1型糖尿病女性的妊娠与循环因子的诸多变化有关，但这些变化并未在脐带血中体现。观察到的营养和炎症介质变化可能与不良妊娠结局有关，并有可能纳入1型糖尿病女性妊娠并发症的预测模型。关键词 1型糖尿病 “血清” “血浆” “脐带血” “妊娠并发症”
