Hodbod T, Szmicsekova K, Cinakova A, Stefikova K, Krivosikova Z, Kralova E, Hrabovska A
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University Bratislava, Slovak Republic.
Physiol Res. 2025 Jul 23;74(3):471-480. doi: 10.33549/physiolres.935558.
Butyrylcholinesterase (BChE) has recently been associated with metabolic imbalance. A correlation between plasma activity and lipid and glucose metabolism has been reported in animal models and human patients. Here, we investigated plasma BChE activity in a rat model of comorbid hypertension and type 1 diabetes mellitus (DM) induced by a single injection of streptozotocin (STZ, 55 mg/kg) in male spontaneously hypertensive rats (SHR) (SHR+DM). The SHR+DM animals exhibit the main characteristics of the human comorbid pathology, including hypertension and hyperglycemia. Although STZ lowered blood pressure in SHR, the animals remained hypertensive as compared to the Wistar controls. Plasma levels of triacylglycerols, cholesterol and HDL were increased, while markers of liver damage such as ALT, AST, were increased and albumin was decreased. Plasma BChE activities were similar in Wistar and SHR. In SHR+DM, plasma BChE activity was increased by 43 %. Interestingly, liver BChE activity and relative mRNA expression were decreased by 60 % in SHR and SHR+DM. While plasma BChE activity is often used as a clinical marker of liver injury, our results suggest that it may not be a reliable indicator. Key words Butyrylcholinesterase " Streptozotocin " Spontaneously hypertensive rats " Diabetes mellitus " Liver damage.
丁酰胆碱酯酶（BChE）最近被认为与代谢失衡有关。在动物模型和人类患者中，已报道血浆活性与脂质和葡萄糖代谢之间存在相关性。在此，我们研究了雄性自发性高血压大鼠（SHR）单次注射链脲佐菌素（STZ，55mg/kg）诱导的高血压合并1型糖尿病（DM）大鼠模型（SHR+DM）中的血浆BChE活性。SHR+DM动物表现出人类合并症病理的主要特征，包括高血压和高血糖。尽管STZ降低了SHR的血压，但与Wistar对照组相比，这些动物仍处于高血压状态。血浆甘油三酯、胆固醇和高密度脂蛋白水平升高，而肝损伤标志物如ALT、AST升高，白蛋白降低。Wistar和SHR的血浆BChE活性相似。在SHR+DM中，血浆BChE活性增加了43%。有趣的是，SHR和SHR+DM的肝脏BChE活性和相对mRNA表达降低了60%。虽然血浆BChE活性常被用作肝损伤的临床标志物，但我们的结果表明它可能不是一个可靠的指标。关键词 丁酰胆碱酯酶 “链脲佐菌素” “自发性高血压大鼠” “糖尿病” “肝损伤”
