Roncaglia-Pereira Victor Augusto, Dumard Carlos Henrique, Monteiro-Machado Marcos, Melo Paulo A, Fonseca Juliana, Meirelles Leonardo, Cunha-Ribeiro Luis, Souza Patrícia, da Silva Jerson Lima, Castilho Leda, de Oliveira Andréa Cheble, Gomes Andre Marco de Oliveira, Strauch Marcelo Abrahão
Institute of Medical Biochemistry Leopoldo de Meis and National Institute of Science and Technology for Structural Biology and Bioimaging, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratório de Farmacologia das Toxinas, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Vet Med Sci. 2025 Sep;11(5):e70488. doi: 10.1002/vms3.70488.
In this study, we aim to report the persistent presence of anti-SARS-CoV-2 immunoglobulins in pre-immunized mare offspring. Three mares from Vital Brazil Institute were pre-immunized with recombinant SARS-CoV-2 spike protein and became pregnant during this period. After parturition, the mares' serum and colostrum/milk and foal serum were collected over 6 weeks. Our results have shown high and persistent presence of IgG and neutralizing antibodies over the weeks not only in the mares' serum, as expected, but in mares' colostrum/milk and foal serum as well-what were very surprising. This peculiar approach, which we were able to track specifically antibodies produced from an antigen inoculated only in the pregnant mares', is distinct from the usual methodology applied in the current reports in this field. Thus, together, these data and our methodology could lead to new approaches to better understanding about equine passive immunization by newborn's breast-feeding.
在本研究中，我们旨在报告在未免疫母马的后代中抗SARS-CoV-2免疫球蛋白的持续存在情况。来自巴西生命研究所的三匹母马用重组SARS-CoV-2刺突蛋白进行了预免疫，并在此期间怀孕。分娩后，在6周内收集了母马的血清、初乳/乳汁以及马驹血清。我们的结果显示，不仅在母马血清中，如预期的那样，在数周内IgG和中和抗体含量高且持续存在，而且在母马的初乳/乳汁和马驹血清中也是如此，这非常令人惊讶。我们能够专门追踪仅在怀孕母马中接种抗原后产生的抗体，这种独特的方法不同于该领域当前报告中使用的常规方法。因此，这些数据和我们的方法共同可能会带来新的途径，以更好地理解新生马驹通过母乳喂养进行的被动免疫。
