Xu Zhe, He Huaiyue, Wang Dan, Xiao Mengmeng, Li Jinman, Hou Yulin, Chen Yangyi, Zu Xuan, Hu Jingtao, Yang Guilian, Jiang Yanlong, Yang Wentao, Huang Haibin, Shi Chunwei, Zeng Yan, Wang Nan, Cao Xin, Wang Jianzhong, Wang Chunfeng
College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, China.
College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, China.
Vet Microbiol. 2025 Sep;308:110651. doi: 10.1016/j.vetmic.2025.110651. Epub 2025 Jul 20.
Canine parvovirus type 2 (CPV-2) causes an acute and highly transmissible disease characterized by severe hemorrhagic enteritis and myocarditis, ultimately resulting in the death of dogs and wild carnivores. At present, although vaccines have been developed for CPV-2, there are no specific and effective vaccines available for prevention of CPV-2c infection. To develop mucosal vaccines against CPV-2c, we constructed recombinant Lactobacillus plantarum (L. plantarum) expressing the VP2 antigen of CPV-2c. We amplified the VP2 gene from a CPV-2c subtype strain and subsequently constructed recombinant L. plantarum NC8 strains that express the VP2 and pgsA'-VP2 proteins, respectively. This work has shown that recombinant L. plantarum strains NC8/pSIP409-VP2 and NC8/pSIP409-pgsA'-VP2 can effectively activate dendritic cells maturation markers (CD80, CD86, and MHC-Ⅱ) while inducing robust IgG and IFN-γ responses in both mice and dogs. Furthermore, recombinant L. plantarum elevated sIgA and IgG concentrations and augmented B220⁺IgA⁺ cell populations. The data indicated that the recombinant L. plantarum developed in this work can significantly enhance the host immunity as an oral vaccination and may contribute to the prevention and control of canine parvovirus infection in dogs.
犬细小病毒2型（CPV-2）可引发一种急性且传染性极强的疾病，其特征为严重的出血性肠炎和心肌炎，最终导致犬类和野生食肉动物死亡。目前，尽管已经研发出针对CPV-2的疫苗，但尚无用于预防CPV-2c感染的特异性有效疫苗。为研发针对CPV-2c的黏膜疫苗，我们构建了表达CPV-2c的VP2抗原的重组植物乳杆菌（L. plantarum）。我们从一株CPV-2c亚型毒株中扩增出VP2基因，随后构建了分别表达VP2和pgsA'-VP2蛋白的重组植物乳杆菌NC8菌株。这项研究表明，重组植物乳杆菌菌株NC8/pSIP409-VP2和NC8/pSIP409-pgsA'-VP2在诱导小鼠和犬产生强烈的IgG和IFN-γ应答的同时，能够有效激活树突状细胞成熟标志物（CD80、CD86和MHC-Ⅱ）。此外，重组植物乳杆菌提高了sIgA和IgG的浓度，并增加了B220⁺IgA⁺细胞群体。数据表明，本研究中研发的重组植物乳杆菌作为口服疫苗可显著增强宿主免疫力，可能有助于犬细小病毒感染的预防和控制。
