Chang H C, Jones O W
Prenat Diagn. 1985 Sep-Oct;5(5):305-12. doi: 10.1002/pd.1970050502.
Reduction in serum requirement for culture of primary human amniotic fluid cells can be achieved by the addition of 10 growth-promoting factors to the nutrient medium. This supplemented medium preserves cell types normally found in amniotic fluid cell cultures supplemented with 20-30 per cent fetal bovine serum. The volume of amniotic fluid required to initiate culture can be as little as 1 ml. Amniotic fluid samples contaminated with red blood cells with no visible clot also grow well in the low serum medium. Cell-free amniotic fluid combined with equal parts of supplemented medium is useful in initiating cell culture.
通过向营养培养基中添加10种生长促进因子，可降低原代人羊水细胞培养所需的血清量。这种补充培养基可保留在添加20%-30%胎牛血清的羊水细胞培养物中通常发现的细胞类型。启动培养所需的羊水体积可低至1毫升。被红细胞污染且无可见凝块的羊水样本在低血清培养基中也能良好生长。无细胞羊水与等量的补充培养基混合，有助于启动细胞培养。
