Weissbach A, Nalin C M, Ward C A, Bolden A H
Prog Clin Biol Res. 1985;198:79-94.
The HeLa DNA methylase can methylate selected cytosine residues in oligodeoxynucleotides as small as 12-16 nucleotides in length in vitro. The maximum methylation rate seems to require oligomers having more than one C-G in the molecule even when only one of the C-G pairs is methylated. Compounds which contain a high G+C content also seem to be favored substrates. The use of defined synthetic oligodeoxynucleotides permits one to demonstrate that flanking DNA sequences can be critical in determining whether a C-G site can be methylated.
海拉细胞DNA甲基化酶在体外能够甲基化长度仅为12 - 16个核苷酸的寡脱氧核苷酸中选定的胞嘧啶残基。即使分子中只有一个C-G对被甲基化，最大甲基化率似乎也需要分子中含有多个C-G的寡聚物。含有高G+C含量的化合物似乎也是理想的底物。使用特定的合成寡脱氧核苷酸能够证明侧翼DNA序列在决定C-G位点是否能够被甲基化方面可能至关重要。
