Wang Xiaoming, Ma Junjun, Fang Chaoxian, Zhu Jinghuan, Wang Shilong, Yang Zuli
Key Laboratory for the Green and Efficient Production Technology of Sugarcane, Guangxi Science & Technology Normal University, Laibin, China.
Institute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical & Chemical Analysis), Beijing, China.
Front Microbiol. 2025 Jul 9;16:1541329. doi: 10.3389/fmicb.2025.1541329. eCollection 2025.
Rhizosphere ecological factors play a crucial role in the soil feedback function of ratoon sugarcane. However, limited information exists regarding the differences and relationships among these factors across various soil types (sandy, loam, and clay) and their impact on sugarcane growth and yield characteristics. This study aims to address this knowledge gap by investigating the rhizosphere ecological dynamics of ratoon sugarcane in different soil types.
A combination of biochemical experiments and high-throughput sequencing was employed to analyze the rhizosphere ecological factors of ratoon sugarcane. The study examined biochemical function- and enzymatic activity-related factors in the rhizosphere soil, as well as the microbial community composition and their relationships with sugarcane growth and yield characteristics.
Biochemical function- and enzymatic activity-related factors in the rhizosphere soil were generally positively correlated with each other and with sugarcane growth characteristics. For instance, soil respiration and soil catalase activity showed significant positive correlations with theoretical sugarcane yield (correlation coefficients of 0.773, < 0.05 and 0.863, < 0.01, respectively). Symbiotic relationships were observed between the rhizosphere soil microbial and root system endophyte communities. Functional differences in microbial communities among different soil types were significant ( < 0.05), indicating that soil type strongly influences the functional expression of rhizosphere soil microbial communities. The abundance of bacteria and fungi in the ratoon sugarcane rhizosphere soil was negatively and positively correlated with most soil biochemical functions, respectively. Additionally, correlations existed between the abundance of endophytic bacteria and fungi in the root system and sugarcane yield. Eleven soil biochemical and functional factors were positively correlated with theoretical sugarcane yield and theoretical sugar yield indicators.
Our findings suggest that different feedback directions and correlation strengths exist between sugarcane growth characteristics and various ecological factors in their rhizosphere across different soil types and spatial scales. These results provide insights into the complex interactions between sugarcane and its rhizosphere environment, highlighting the importance of soil type in shaping these interactions. The study offers guidance for improving soil microbial community structure to enhance sugarcane growth and yield, serving as a valuable reference for soil management in sugarcane fields.
根际生态因子在宿根甘蔗的土壤反馈功能中起着关键作用。然而，关于这些因子在不同土壤类型（砂土、壤土和黏土）之间的差异和关系及其对甘蔗生长和产量特性的影响，现有信息有限。本研究旨在通过调查不同土壤类型中宿根甘蔗的根际生态动态来填补这一知识空白。
采用生化实验和高通量测序相结合的方法分析宿根甘蔗的根际生态因子。该研究检测了根际土壤中与生化功能和酶活性相关的因子，以及微生物群落组成及其与甘蔗生长和产量特性的关系。
根际土壤中与生化功能和酶活性相关的因子通常彼此之间以及与甘蔗生长特性呈正相关。例如，土壤呼吸和土壤过氧化氢酶活性与理论甘蔗产量呈显著正相关（相关系数分别为0.773，<0.05和0.863，<0.01）。在根际土壤微生物群落和根系内生菌群落之间观察到共生关系。不同土壤类型之间微生物群落的功能差异显著（<0.05），表明土壤类型强烈影响根际土壤微生物群落的功能表达。宿根甘蔗根际土壤中细菌和真菌的丰度分别与大多数土壤生化功能呈负相关和正相关。此外，根系内生细菌和真菌的丰度与甘蔗产量之间存在相关性。11个土壤生化和功能因子与理论甘蔗产量和理论蔗糖产量指标呈正相关。
我们的研究结果表明，在不同土壤类型和空间尺度上，甘蔗生长特性与其根际各种生态因子之间存在不同的反馈方向和相关强度。这些结果为了解甘蔗与其根际环境之间的复杂相互作用提供了见解，突出了土壤类型在塑造这些相互作用中的重要性。该研究为改善土壤微生物群落结构以促进甘蔗生长和提高产量提供了指导，为甘蔗田土壤管理提供了有价值の参考。
