Parry Ruhban Ansar, Wani Sajad Hamid, Mir Irfan Ahmad, Bhat Basharat Ahmad, Hussain Mahboob Ul, Mir Mushtaq Ahmad, Bashir Nasreena, Fadul Abdalla N, Jangra Surender, Vats Sharad, Ganie Showkat Ahmad
Department of Clinical Biochemistry, University of Kashmir, Srinagar, India.
Department of Bioscience & Biotechnology, Banasthali Vidyapith, Tonk, Rajasthan, India.
Front Pharmacol. 2025 Jul 9;16:1595604. doi: 10.3389/fphar.2025.1595604. eCollection 2025.
Inflammation plays a critical role in colon carcinogenesis by dysregulating multiple signalling pathways. Targeting these inflammatory pathways is essential for effective colorectal cancer management. This study aims to investigate how L. extracts can prevent inflammation-related colorectal cancer both and .
Anti-inflammatory assays were conducted using standard protocols. Anticancer activity was evaluated by MTT assay, while protein expression was analysed via Western blotting. Metabolite identification was performed using GC-MS analysis. experiments were carried out in BALB/c mice, including histopathological evaluations and biochemical assays, to assess the physiological and molecular effects of the extracts. All experimental procedures followed established scientific guidelines to ensure accuracy and reliability of the results.
assays revealed that extracts inhibited protein denaturation, nitric oxide production, and membrane hemolysis with IC values ranging from 47.46 to 268.46 μg/mL. MTT assays demonstrated potent cytotoxicity against HCT116 (IC = 30.94 μg/mL), HT29 (IC = 46.89 μg/mL), and SW480 (IC = 63.40 μg/mL) cell lines. The extracts significantly downregulated COX-2, NFκB, and PPAR-γ protein levels and induced PARP and Caspase 3 cleavage. GC-MS analysis identified anti-inflammatory and anticancer metabolites, including kaempferol derivatives, α-Tocopherol, and phytol. , AR-EA and AR-Met extracts attenuated LPS-induced paw edema and restored altered biochemical parameters in mice models, highlighting the extracts' therapeutic potential against inflammation-associated colorectal cancer.
The findings highlight the therapeutic potential of extracts as natural anti-inflammatory and anticancer agents, offering a promising avenue for purification of metabolites which can be utilised for the prevention and management of inflammation-associated colorectal cancer.
炎症通过失调多种信号通路在结肠癌发生过程中起关键作用。靶向这些炎症通路对于有效的结直肠癌管理至关重要。本研究旨在探究L.提取物如何在体内和体外预防炎症相关的结直肠癌。
使用标准方案进行抗炎试验。通过MTT试验评估抗癌活性，同时通过蛋白质印迹分析蛋白质表达。使用气相色谱 - 质谱分析进行代谢物鉴定。在BALB/c小鼠中进行体内实验，包括组织病理学评估和生化测定，以评估提取物的生理和分子效应。所有实验程序均遵循既定的科学指南，以确保结果的准确性和可靠性。
体外试验表明，提取物抑制蛋白质变性、一氧化氮产生和膜溶血，IC值范围为47.46至268.46μg/mL。MTT试验证明对HCT116（IC = 30.94μg/mL）、HT29（IC = 46.89μg/mL）和SW480（IC = 63.40μg/mL）细胞系具有强大的细胞毒性。提取物显著下调COX - 2、NFκB和PPAR - γ蛋白水平，并诱导PARP和Caspase 3裂解。气相色谱 - 质谱分析鉴定出抗炎和抗癌代谢物，包括山奈酚衍生物、α - 生育酚和叶绿醇。体内实验中，AR - EA和AR - Met提取物减轻了LPS诱导的爪肿胀，并恢复了小鼠模型中改变的生化参数，突出了提取物对炎症相关结直肠癌的治疗潜力。
这些发现突出了提取物作为天然抗炎和抗癌剂的治疗潜力，为可用于预防和管理炎症相关结直肠癌的代谢物纯化提供了一条有前景的途径。
