Sato C S, Gyorkey F
Clin Chim Acta. 1977 Aug 1;78(3):365-9. doi: 10.1016/0009-8981(77)90069-9.
Using the modified bidimensional electrophoresis method, characteristic patterns of urinary glycosaminoglycans were obtained for nine different syndromes of mucopolysaccharidoses and normal. Each pattern, portrayed on a 55 mm square cellulose acetate membrane, was visualized by staining the electrophoretically separated GAGs with alcian blue. The method, tested with 38 cases, was precise and sensitive; the first direction of electrophoresis was run for 25 min and the second direction for 60 min. Because the method is qualitative, the usual quantitative measurements and calculations are circumvented. Moreover, any irregular pattern can readily be verified by repeating the bidimensional electrophoresis. Thus, the clarity of the pattern itself offers assurance of its diagnostic reliabilty; consequently, false negative and false positive results should be minimized.
采用改良的双向电泳法，获得了九种不同类型黏多糖贮积症及正常情况的尿糖胺聚糖特征图谱。每个图谱描绘在55平方毫米的醋酸纤维素膜上，通过用阿尔辛蓝对电泳分离的糖胺聚糖进行染色来显示。该方法经38例测试，精确且灵敏；第一向电泳运行25分钟，第二向电泳运行60分钟。由于该方法是定性的，避免了常规的定量测量和计算。此外，通过重复双向电泳可以很容易地验证任何不规则图谱。因此，图谱本身的清晰度保证了其诊断可靠性；从而应尽量减少假阴性和假阳性结果。
