Zhu Rui, Jiang Tao
Orthopedics, Changzhou Hospital Affiliated to Nanjing University of Chinese Medicine, No. 25, Heping North Road, Tianning District, Changzhou City, 213000, Jiangsu Province, China.
Biochem Genet. 2025 Jul 24. doi: 10.1007/s10528-025-11206-z.
Fractures are common in osteoporosis (OP) patients. To discover new biomarkers for reducing the incidence of fractures by taking preventive measures. Hsa_circ_0086004 and hsa-miR-665 were screened using the GEO and starBase databases. Their diagnostic and prognostic value in osteoporotic fractures was evaluated by constructing ROC curves. An osteogenic differentiation medium (OM) was used to induce osteogenic differentiation of human mesenchymal stem cells (hMSCs). Transfected hsa_circ_0086004 plasmid to detect its regulation on hsa-miR-665 and effects on the function of osteoblasts and osteogenic differentiation, and the hsa-miR-665 role in which was determined by transfecting its inhibitor and mimic. Hsa_circ_0086004 was deficient in the serum of OP patients, and its level was further decreased with the occurrence of fractures. Hsa-miR-665 was opposite. Overexpression of hsa_circ_0086004 and inhibition of hsa-miR-665 promoted the growth of osteoblasts and facilitated the osteogenic differentiation of hMSCs. Transfection of hsa_circ_0086004 plasmid significantly decreased the cellular level of hsa-miR-665. The combination of hsa_circ_0086004 and hsa-miR-665 could predict the occurrence and healing of fracture in OP patients, with AUC of 0.907 and 0.902, respectively. Hsa_circ_0086004 may promote fracture healing in OP patients by inhibiting hsa-miR-665, and they are potential biomarkers of OP.
骨折在骨质疏松症（OP）患者中很常见。为了通过采取预防措施发现降低骨折发生率的新生物标志物。利用GEO和starBase数据库筛选了hsa_circ_0086004和hsa-miR-665。通过构建ROC曲线评估它们在骨质疏松性骨折中的诊断和预后价值。使用成骨分化培养基（OM）诱导人间充质干细胞（hMSCs）的成骨分化。转染hsa_circ_0086004质粒以检测其对hsa-miR-665的调控以及对成骨细胞功能和成骨分化的影响，并通过转染其抑制剂和模拟物来确定hsa-miR-665在其中的作用。hsa_circ_0086004在OP患者血清中缺乏，并且随着骨折的发生其水平进一步降低。hsa-miR-665则相反。hsa_circ_0086004的过表达和hsa-miR-665的抑制促进了成骨细胞的生长并促进了hMSCs的成骨分化。hsa_circ_0086004质粒转染显著降低了hsa-miR-665的细胞水平。hsa_circ_0086004和hsa-miR-665的联合可预测OP患者骨折的发生和愈合，AUC分别为0.907和0.902。hsa_circ_0086004可能通过抑制hsa-miR-665促进OP患者的骨折愈合，它们是OP的潜在生物标志物。
