自由活动的HIV-1 Tat转基因小鼠内侧前额叶皮质神经元的GCaMP表达及体内钙成像实验方案。
Protocol for GCaMP expression and in vivo calcium imaging of medial prefrontal cortex neurons in freely behaving HIV-1 Tat transgenic mice.
作者信息
Yadav-Samudrala Barkha J, Ravula Havilah P, Fitting Sylvia
机构信息
Department of Psychology and Neuroscience, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Department of Psychology and Neuroscience, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
出版信息
STAR Protoc. 2025 Jul 22;6(3):103958. doi: 10.1016/j.xpro.2025.103958.
Advancements in imaging techniques have enhanced our ability to study brain structure and function. Genetically encoded calcium indicators enable high-resolution visualization of neuronal activity at the cellular level. Here, we present a protocol for imaging medial prefrontal cortex (mPFC) neurons in a freely behaving neuroHIV mouse model. We describe steps for survival surgery preparation, craniotomy, durotomy, and adeno-associated virus (AAV) injection. We then detail procedures for implanting the gradient-refractive-index (GRIN) lens, building a headcap, and checking viral expression. For complete details on the use and execution of this protocol, please refer to Yadav-Samudrala et al..
成像技术的进步提高了我们研究大脑结构和功能的能力。基因编码钙指示剂能够在细胞水平上对神经元活动进行高分辨率可视化。在此，我们展示了一种在自由活动的神经艾滋病小鼠模型中对内侧前额叶皮质（mPFC）神经元进行成像的方案。我们描述了生存手术准备、开颅术、硬脑膜切开术和腺相关病毒（AAV）注射的步骤。然后，我们详细介绍了植入梯度折射率（GRIN）透镜、构建头帽以及检查病毒表达的程序。有关此方案的使用和执行的完整详细信息，请参考Yadav-Samudrala等人的研究。
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