Mori H, Shiba K, Tsuji S, Matsuda H, Hisada K, Kojima K
Radioisotopes. 1985 Jul;34(7):359-63. doi: 10.3769/radioisotopes.34.7_359.
The kinetic and pharmacological characteristics of 3H-spiroperidol binding sites were studied in slide mounted sections of rat forebrain, and optical binding conditions were defined. Using the receptor macroautoradiographic techniques with tritium-sensitive LKB sheet film, the distribution of dopamine (D2) receptor was determined in slices including striatum of rat brain. The autoradiograms were analyzed using Video Digitizer System combined with video camera and minicomputer, and the subtraction images were obtained. These studies suggest that this quantitative receptor macroautoradiography might be useful in the explanation of etiology in the field of neuro-psychiatric diseases and the fundamental studies of positron emission computed tomography, since this method has several advantages over in vivo autoradiography and in vitro receptor assay.
在大鼠前脑的载玻片标本上研究了3H-螺哌啶醇结合位点的动力学和药理学特性，并确定了光学结合条件。使用对氚敏感的LKB薄层胶片的受体宏观放射自显影技术，测定了包括大鼠脑纹状体在内的切片中多巴胺（D2）受体的分布。使用结合了摄像机和小型计算机的视频数字化系统分析放射自显影片，并获得减法图像。这些研究表明，这种定量受体宏观放射自显影术可能有助于解释神经精神疾病领域的病因以及正电子发射计算机断层扫描的基础研究，因为该方法相对于体内放射自显影术和体外受体测定法具有若干优势。
