Zheng Ya-Mei, Ye Meng-Meng, Zhang Hong-Ying, Luo Dan-Ping, Liu Tao, He Xing-Xiang, Chen Xian-Yun, Wu Li-Hao
Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, China.
Department of Gastroenterology, The First People's Hospital of Shaoguan, Shaoguan, Guangdong, China.
Front Cell Infect Microbiol. 2025 Jul 10;15:1606417. doi: 10.3389/fcimb.2025.1606417. eCollection 2025.
Autism spectrum disorder (ASD) is a serious neurodevelopmental disorder with no effective treatment. This study explored the short-term clinical effects of washed microbiota transplantation (WMT) with different numbers of donors on autism.
Consecutive ASD patients treated with two continuous WMT courses from March 2020 to March 2022 at the First Affiliated Hospital of Guangdong Pharmaceutical University were retrospectively assessed. Basic information, aberrant behavior checklist (ABC) scores, childhood autism rating scale (CARS) scores, sleep disturbance scale for children (SDSC) scores, adverse reactions, and feces were collected.
Forty-four patients were included (single-donor group: 17 patients; multidonor group: 27 patients). The CARS, ABC and SDSC scores didn't differ between the two groups before treatment. After two courses, the scores for the 44 patients were lower than those at baseline (P<0.05), with no severe adverse reactions observed. After the first course, the mean ABC (P=0.049) and SDSC (P=0.019) scores were significantly different between the single-donor and multidonor groups, but the difference disappeared after two courses. The alpha-diversity of the faecal flora in the effective-group was greater than that in the ineffective-group (Shannon index P=0.0018). was the predominant genus in the effective group, whereas , and were predominant genera in the ineffective group.
After two WMT courses, the symptoms of ASD improved, with good short-term treatment efficacy. The ASD symptom improvement did not differ between the single-donor and multidonor groups. Changes in the alpha-diversity and abundance of the faecal microbiota after WMT may be related to treatment efficacy.
自闭症谱系障碍（ASD）是一种严重的神经发育障碍，尚无有效治疗方法。本研究探讨了不同供体数量的洗涤菌群移植（WMT）对自闭症的短期临床效果。
回顾性评估2020年3月至2022年3月在广东药科大学附属第一医院接受两个连续WMT疗程治疗的连续性ASD患者。收集基本信息、异常行为检查表（ABC）评分、儿童自闭症评定量表（CARS）评分、儿童睡眠障碍量表（SDSC）评分、不良反应和粪便。
纳入44例患者（单供体组：17例患者；多供体组：27例患者）。两组治疗前CARS、ABC和SDSC评分无差异。两个疗程后，44例患者的评分低于基线水平（P<0.05），未观察到严重不良反应。第一个疗程后，单供体组和多供体组的平均ABC评分（P=0.049）和SDSC评分（P=0.019）有显著差异，但两个疗程后差异消失。有效组粪便菌群α多样性大于无效组（香农指数P=0.0018）。 是有效组中的优势菌属，而 、 和 是无效组中的优势菌属。
两个WMT疗程后，ASD症状改善，短期治疗效果良好。单供体组和多供体组ASD症状改善无差异。WMT后粪便微生物群α多样性和丰度的变化可能与治疗效果有关。
