Li Lina, Yang Liuqing, Wang Yuzhen, Nan Kejun
Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Department of Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, Shaanxi, China.
Front Genet. 2025 Jul 10;16:1594354. doi: 10.3389/fgene.2025.1594354. eCollection 2025.
Recent studies have shown that circular RNA (circRNA) plays an important role in the development of gastric cancer. However, despite the widespread use of high-throughput sequencing technologies, the function of many circRNAs in gastric cancer remains unclear. In this study, we selected a circ_0002082 (circ-malat1) that is differentially expressed between normal gastric epithelial cells and gastric cancer cells to further investigate its role and molecular mechanisms in regulating gastric cancer development. The study primarily explores the function and molecular mechanisms of circ-malat1 at the cellular and molecular levels. Functional studies reveal that overexpression of circ-malat1 promotes gastric cancer cell growth. Conversely, silencing the expression of circ-malat1 has the opposite effect. Mechanistic studies indicate that circ-malat1 is predominantly expressed in the cytoplasm of gastric cancer cells and can act as a competing endogenous RNA by sequestering miR-154-5p, thereby enhancing CCND2 gene expression. In conclusion, circ-malat1 promotes the development of gastric cancer by competitively binding to miR-154-5p. Based on literature reports, it is speculated that circ-malat1 may also participate in regulation through other pathways, which require further investigation.
最近的研究表明，环状RNA（circRNA）在胃癌的发展中起重要作用。然而，尽管高通量测序技术已被广泛应用，但许多circRNA在胃癌中的功能仍不清楚。在本研究中，我们选择了一种在正常胃上皮细胞和胃癌细胞之间差异表达的circ_0002082（circ-malat1），以进一步研究其在调节胃癌发展中的作用和分子机制。该研究主要在细胞和分子水平上探索circ-malat1的功能和分子机制。功能研究表明，circ-malat1的过表达促进胃癌细胞生长。相反，沉默circ-malat1的表达则产生相反的效果。机制研究表明，circ-malat1主要在胃癌细胞的细胞质中表达，并可通过隔离miR-154-5p作为竞争性内源性RNA，从而增强CCND2基因的表达。总之，circ-malat1通过与miR-154-5p竞争性结合促进胃癌的发展。根据文献报道，推测circ-malat1也可能通过其他途径参与调节，这需要进一步研究。
