Bova Stefania, Faggiano Serena, De Bei Omar, Marchetti Marialaura, Bruno Stefano, Campanini Barbara, Bettati Stefano, Ronda Luca
SITEIA.PARMA, University of Parma, 43124 Parma, Italy.
Department of Food and Drug, University of Parma, 43124 Parma, Italy.
Biosensors (Basel). 2025 Jul 3;15(7):425. doi: 10.3390/bios15070425.
In the pharmaceutical field, lipid-based nanoparticles are extensively used for drug or vaccine delivery, particularly for treating respiratory disorders. However, their physico-chemical instability, particularly associated with lipid degradation through hydrolysis or oxidation, can affect their encapsulation properties. To monitor the stability of lipid-based formulations over time, we prepared acrylodan-labeled bovine serum albumin (here called albuminodan), and showed it is a fluorescent biosensor capable of concomitantly detect phospholipids as well as their degradation products, i.e., fatty acids and lysophospholipids. We demonstrated that this tool can be used to follow the distribution of lipids in an aqueous phase and hence could be suitable to characterize the hydrolysis of phospholipids in a lipid-based formulation to monitor the stability of nanoparticles.
在制药领域，脂质纳米颗粒被广泛用于药物或疫苗递送，特别是用于治疗呼吸系统疾病。然而，它们的物理化学不稳定性，尤其是与通过水解或氧化导致的脂质降解相关的不稳定性，会影响其包封性能。为了监测脂质制剂随时间的稳定性，我们制备了丙烯酰罗丹标记的牛血清白蛋白（以下称为白蛋白罗丹），并表明它是一种荧光生物传感器，能够同时检测磷脂及其降解产物，即脂肪酸和溶血磷脂。我们证明了该工具可用于跟踪脂质在水相中的分布，因此可能适用于表征脂质制剂中磷脂的水解，以监测纳米颗粒的稳定性。
