Terenteva Sophie, Golani-Zaidie Linoy, Avivi Shira, Lustig Yaniv, Indenbaum Victoria, Koren Ravit, Hoa Tran Mai, Tuyen Tong Thi Kim, Huyen Ma Thi, Hoan Nguyen Minh, Hoi Le Thi, Trung Nguyen Vu, Schwartz Eli, Danielli Amos
Faculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan 5290002, Israel.
Central Virology Laboratory, Israel Ministry of Health, Chaim Sheba Medical Centre, Tel-HaShomer, Ramat Gan 5262000, Israel.
Biosensors (Basel). 2025 Jul 14;15(7):453. doi: 10.3390/bios15070453.
Dengue virus (DENV) poses a major global health concern, with over 6.5 million cases and 7300 deaths reported in 2023. Accurate serological assays are essential for tracking infection history, evaluating disease severity, and guiding vaccination strategies. However, existing assays are limited in their specificity, sensitivity, and cross-reactivity. Using optical modulation biosensing (OMB) technology and non-structural protein 1 (NS1) antigens from DENV-1-3, we developed highly sensitive and quantitative serotype-specific anti-DENV NS1 IgG serological assays. The OMB-based assays offered a wide dynamic range (4-log), low detection limits (400 ng/L), fast turnaround (1.5 h), and a simplified workflow. Using samples from endemic (Vietnam) and non-endemic (Israel) regions, we assessed intra-DENV and inter- cross-reactivity. Each assay detected DENV infection with a 100% sensitivity for the corresponding serotype and 64% to 90% for other serotypes. Cross-reactivity with Zika, Japanese encephalitis, and West Nile viruses ranged from 21% to 65%, reflecting NS1 antigen conservation. Our study provides valuable insights into the cross-reactivity of DENV NS1 antigens widely used in research and highlights the potential of OMB-based assays for quantitative and epidemiological studies. Ongoing efforts should aim to minimize cross-reactivity while maintaining sensitivity and explore integration with complementary platforms for improved diagnostic precision.
登革病毒（DENV）是全球主要的健康问题，2023年报告的病例超过650万例，死亡7300人。准确的血清学检测对于追踪感染史、评估疾病严重程度和指导疫苗接种策略至关重要。然而，现有的检测方法在特异性、敏感性和交叉反应性方面存在局限性。我们利用光调制生物传感（OMB）技术和来自DENV-1-3的非结构蛋白1（NS1）抗原，开发了高度敏感且定量的血清型特异性抗DENV NS1 IgG血清学检测方法。基于OMB的检测方法具有宽动态范围（约4个对数）、低检测限（约400 ng/L）、快速周转时间（1.5小时）和简化的工作流程。我们使用来自流行地区（越南）和非流行地区（以色列）的样本，评估了DENV内部和交叉反应性。每种检测方法对相应血清型的DENV感染检测灵敏度为100%，对其他血清型的检测灵敏度为64%至90%。与寨卡病毒、日本脑炎病毒和西尼罗河病毒的交叉反应率在21%至65%之间，反映了NS1抗原的保守性。我们的研究为广泛用于研究的DENV NS1抗原的交叉反应性提供了有价值的见解，并突出了基于OMB的检测方法在定量和流行病学研究中的潜力。正在进行的努力应旨在在保持灵敏度的同时尽量减少交叉反应性，并探索与互补平台整合以提高诊断精度。
