Lima William Gustavo, Laia Rayssa Maria Rodrigues, Brito Julio Cesar Moreira, Michel Daniel Augusto Guedes Reis, Verly Rodrigo Moreira, Resende Jarbas Magalhães, de Lima Maria Elena
Faculdade Santa Casa de Belo Horizonte, Belo Horizonte 30110-005, Brazil.
Fundação Ezequiel Dias (FUNED), Belo Horizonte 30510-010, Brazil.
Toxins (Basel). 2025 Jun 25;17(7):325. doi: 10.3390/toxins17070325.
can acquire resistance mechanisms to colistin and present a pan-resistant phenotype. Therefore, new alternative agents are imperative to control this pathogen, and the peptide Jelleine-I stands out as a promising prototype. Here, the antibacterial activity of Jelleine-I against clinical isolates of colistin-resistant (CRKP) was investigated. Antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time kill-curve assay. The release of 260 nm-absorbing materials (DNA/RNA) and the release of proteins were used in the lysis assay. Anti-biofilm activity was studied in microplates. In vivo activity was determined by the lethality assay using larvae. The results show that the MIC of Jelleine-I ranged from 16 to 128 µM and the MBC was on average 128 µM. Jelleine-I at 200 µM killed all CRKP cells in suspension (10 colony-forming units (CFU)/mL) after 150 min of incubation. Jelleine-I acts on the CRKP cell membrane inducing lysis. Biomass and viability of CRKP-induced biofilms are reduced after treatment with Jelleine-I, and the use of this peptide in larvae infected with CRKP reduces lethality and improves overall larval health. In conclusion, Jelleine-I is a potential prototype for the development of new antimicrobial agents.
可获得对黏菌素的耐药机制并呈现泛耐药表型。因此，新型替代药物对于控制这种病原体至关重要，而肽Jelleine-I作为一种有前景的原型脱颖而出。在此，研究了Jelleine-I对耐黏菌素肺炎克雷伯菌（CRKP）临床分离株的抗菌活性。通过测定最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和时间杀菌曲线试验来评估抗菌活性。在溶菌试验中使用260nm吸收物质（DNA/RNA）的释放和蛋白质的释放。在微孔板中研究抗生物膜活性。通过使用幼虫的致死率试验来确定体内活性。结果表明，Jelleine-I的MIC范围为16至128μM，MBC平均为128μM。在孵育150分钟后，200μM的Jelleine-I杀死了悬浮液中所有的CRKP细胞（10个菌落形成单位（CFU）/mL）。Jelleine-I作用于CRKP细胞膜诱导细胞裂解。用Jelleine-I处理后，CRKP诱导的生物膜的生物量和活力降低，并且在感染CRKP的幼虫中使用这种肽可降低致死率并改善幼虫的整体健康状况。总之，Jelleine-I是开发新型抗菌药物的潜在原型。
