Pérez-Santana Carmen G, Cazorla-Rivero Sara E, Jiménez-Alonso Ana A, Rodríguez-Esparragón Francisco, González Martín Jesús María, Henríquez-Cabrera Ruth, Clavo-Varas Bernardino, Rodríguez Grau-Bassas Enrique
Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), Universidad de Las Palmas de Gran Canaria (ULPGC), 35400 Arucas, Spain.
Unidad de Investigación Hospital Universitario de Gran Canaria Dr. Negrín, 35010 Las Palmas de Gran Canaria, Spain.
Vet Sci. 2025 Jun 30;12(7):627. doi: 10.3390/vetsci12070627.
Ten dogs with oral malignant melanoma were evaluated and treated with surgery, of which four dogs were diagnosed with melanotic melanoma and six were diagnosed with amelanotic melanoma. Serum samples from oral malignant melanoma (OMM) were collected at baseline, the day of the surgery, and every 3-4 months, during which time a clinical examination and chest X-rays were performed. Concentrations of GM-CSF, IFN-γ, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, KC-like, MCP-1, and TNFα were quantified. Follow-up samples indicated that after the removal of malignant melanoma, the serum levels of GM-CSF, IFN-γ, MCP-1, IL-18, and IL-2 increased significantly. In contrast, when comparing samples from dogs with OMM to those of patients in remission, the concentrations of IL-7 and MCP-1 were significantly higher in the remission samples than in the OMM samples. Furthermore, when comparing the serum concentrations between the OMM-metastasis samples and those patients in remission, elevated levels of MCP-1 were associated with poorer overall survival due to the development of OMM metastasis. Finally, a comparison of cytokines in the melanotic OMM and amelanotic OMM samples revealed that the amelanotic OMM samples exhibited higher concentrations of IL-6, IL-10, and IL-15 compared to the melanotic OMM samples.
对10只患有口腔恶性黑色素瘤的犬进行了评估并接受手术治疗，其中4只犬被诊断为黑素性黑色素瘤，6只被诊断为无黑素性黑色素瘤。在基线、手术当天以及之后每3 - 4个月采集口腔恶性黑色素瘤（OMM）的血清样本，在此期间进行临床检查和胸部X光检查。对GM - CSF、IFN - γ、IL - 2、IL - 6、IL - 7、IL - 8、IL - 10、IL - 15、IL - 18、IP - 10、KC样、MCP - 1和TNFα的浓度进行了定量分析。随访样本表明，切除恶性黑色素瘤后，GM - CSF、IFN - γ、MCP - 1、IL - 18和IL - 2的血清水平显著升高。相比之下，将患有OMM的犬的样本与缓解期患者的样本进行比较时，缓解期样本中IL - 7和MCP - 1的浓度显著高于OMM样本。此外，在比较OMM转移样本与缓解期患者的血清浓度时，由于OMM转移的发生，MCP - 1水平升高与总体生存率较差相关。最后，对黑素性OMM和无黑素性OMM样本中的细胞因子进行比较发现，与黑素性OMM样本相比，无黑素性OMM样本中IL - 6、IL - 10和IL - 15的浓度更高。
