Kothe N, Eichentopf B, Bonhard K
Surg Gynecol Obstet. 1985 Dec;161(6):563-9.
A new infusion solution prepared from stroma-free, crosslinked and pyridoxalated hemoglobin is described. The characteristics of which have been adapted to the functions of native, intra-erythrocyte hemoglobin. The solution contains 8.5 per cent weight per volume of glutardialdehyde modified hemoglobin with a mean molecular weight (Mn) of 200,000 Dalton. The non-crosslinked fraction constitutes 15 per cent of the total hemoglobin. The p50 of 27 torr at pH 7.4 (plasma pH) corresponds to the value for the intra-erythrocyte hemoglobin at pH 7.25. The oncotic pressure is adjusted to 28 torr by the addition of 2.5 per cent weight per volume of human albumin. The methemoglobin content is less than 5 per cent of the total hemoglobin and remains constant for one year. The relative viscosity of the 8.5 per cent hemoglobin solution is 3.0 and, thus, is lower than the viscosity of blood. The intravascular half-life was determined in chimpanzees as about 16 hours. On the basis of available data, this hemoglobin solution may be suitable for clinical use as oxygen carrying plasma substitute.
本文描述了一种由无基质、交联且吡哆醛化血红蛋白制备的新型输注溶液。其特性已根据天然红细胞内血红蛋白的功能进行了调整。该溶液含有8.5%（重量/体积）戊二醛修饰血红蛋白，平均分子量（Mn）为200,000道尔顿。非交联部分占总血红蛋白的15%。在pH 7.4（血浆pH）时p50为27托，这与pH 7.25时红细胞内血红蛋白的值相对应。通过添加2.5%（重量/体积）的人白蛋白将胶体渗透压调节至28托。高铁血红蛋白含量低于总血红蛋白的5%，且一年内保持恒定。8.5%血红蛋白溶液的相对粘度为3.0，因此低于血液粘度。在黑猩猩体内测定的血管内半衰期约为16小时。根据现有数据，这种血红蛋白溶液可能适合作为携氧血浆代用品用于临床。
