Oyeleye Idowu Sunday, Bolarinde Abidemi Abiola, Ademiluyi Adedayo Oluwaseun, Oboh Ganiyu, Ojo Olajide Raymond
Department of Medical Biochemistry, Federal University of Technology, P.M.B. 704, Akure, 340001, Nigeria; Functional Foods, Nutraceuticals and Phytomedicine Laboratory Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure, 340001, Nigeria.
Functional Foods, Nutraceuticals and Phytomedicine Laboratory Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure, 340001, Nigeria.
J Ethnopharmacol. 2025 Jul 24;353(Pt A):120326. doi: 10.1016/j.jep.2025.120326.
Bitter leaf (Vernonia amygdalina Delile) and Siam weed (Chromolaena odorata (L.) R.M.King & H.Rob.) are herbal plants known for their diverse therapeutic purposes, including the treatment of malaria. However, there is a dearth of information on their ability to reverse the negative impacts of malaria infection on reproduction-linked organs such as the testes.
This study investigated the effect of bitter leaf and Siam weed aqueous extracts in reversing testicular oxidative damage caused by plasmodium berghei Infection in male mice.
The bioactive components of Siam weed and bitter leaf were analyzed. Sixty-four (64) male mice were inoculated with strain NK65 Plasmodium berghei (Chloroquine, CQ sensitive), and the parasitemia suppression was assessed. The mice were treated with bitter leaf and Siam weed aqueous extracts (100, 200, and 400 mg/kg) for five (5) days, with Chloroquine (10 mg/kg) as standard. The reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), antioxidant levels and enzyme activities were assessed on the testes of the mice.
Bitter leaf and Siam weed contain several antioxidants and anti-inflammatory bioactive components. Treatments with these herbs suppressed the parasitemia level, significantly (p < 0.05) reduced ROS, TBARS, and had an inhibitory effect on enzyme activities, with an increase in antioxidant levels in a dose-dependent manner compared to infected, untreated mice.
These findings indicate that bitter leaf and Siam weed aqueous extracts can reverse the negative effects of malaria infection on the testes via modulation of oxidative stress pathways.
苦叶（扁桃斑鸠菊）和飞机草是具有多种治疗用途的草药，包括治疗疟疾。然而，关于它们逆转疟疾感染对睾丸等与生殖相关器官的负面影响的能力，目前缺乏相关信息。
本研究调查了苦叶和飞机草水提取物对逆转伯氏疟原虫感染所致雄性小鼠睾丸氧化损伤的作用。
分析了飞机草和苦叶的生物活性成分。给64只雄性小鼠接种NK65伯氏疟原虫株（对氯喹敏感），并评估疟原虫血症抑制情况。用苦叶和飞机草水提取物（100、200和400mg/kg）对小鼠进行为期5天的治疗，以氯喹（10mg/kg）作为对照。对小鼠睾丸的活性氧（ROS）、硫代巴比妥酸反应性物质（TBARS）、抗氧化水平和酶活性进行了评估。
苦叶和飞机草含有多种抗氧化剂和抗炎生物活性成分。用这些草药进行治疗可抑制疟原虫血症水平，显著（p<0.05）降低ROS、TBARS，并对酶活性有抑制作用，与未治疗的感染小鼠相比，抗氧化水平呈剂量依赖性增加。
这些发现表明，苦叶和飞机草水提取物可通过调节氧化应激途径逆转疟疾感染对睾丸的负面影响。
