Wu Shutian, Huang Jianhua, Shi Lei, Wu Minfeng, Shi Jingjuan, Tao Hui, Wang Xiuli, Li Shanshan, Wang Hongwei
Department of Dermatology, Huadong Hospital, Fudan University, 221 West Yan'an Road, Shanghai, 200040, China.
Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, China.
Lasers Med Sci. 2025 Jul 26;40(1):326. doi: 10.1007/s10103-024-04249-y.
Methicillin-resistant Staphylococcus aureus (MRSA) is the most common and difficult-to-treat pathogen in chronic wounds. In this study, a novel derivative of chlorin, ShengTaiBuFen (STBF), was used as a photosensitiser to investigate the effect of STBF mediated photodynamic therapy (STBF-PDT) on MRSA-infected ulcers. Appropriate incubation time and concentration parameters were initially explored to evaluate the anti-MRSA performance of STBF-PDT in vitro. An infected mouse skin ulcer model was further established to assess the antibacterial ability of STBF-PDT on MRSA in ulcers and the ultimate effect on ulcers. Our data showed that the antibacterial activity of STBF-PDT against planktonic MRSA was strongest at 25 µM concentration in a light dose-dependent way, and MRSA strains were completely eliminated at 120 J/cm. The in vivo study showed the STBF-PDT group had basically healed (96.93 ± 2.21%), while the untreated group (28.83 ± 10.25%), the red light group (36.63 ± 13.08%) and the STBF group (32.95 ± 9.38%) had poor healing. STBF-PDT displayed marked bacteriostatic effect in vivo as opposed to the other three groups. The mean viable bacterial log of wounds subjected to STBF-PDT revealed 6.80 ± 6.98 log cfu/g (a substantial reduction of 99.83% versus the untreated control). STBF was believed to be an advantageous and reliable photosensitizer, and its mediated PDT provided a reliable and safe modality for the clinical treatment of potentially drug-resistant microbial infectious diseases such as chronic wounds.
耐甲氧西林金黄色葡萄球菌（MRSA）是慢性伤口中最常见且最难治疗的病原体。在本研究中，一种新型二氢卟吩衍生物——圣泰部分（STBF），被用作光敏剂，以研究STBF介导的光动力疗法（STBF-PDT）对MRSA感染溃疡的影响。最初探索了合适的孵育时间和浓度参数，以评估STBF-PDT在体外的抗MRSA性能。进一步建立了感染小鼠皮肤溃疡模型，以评估STBF-PDT对溃疡中MRSA的抗菌能力以及对溃疡的最终影响。我们的数据表明，STBF-PDT对浮游MRSA的抗菌活性在浓度为25µM时最强，呈光剂量依赖性，且在120 J/cm时MRSA菌株被完全清除。体内研究表明，STBF-PDT组基本愈合（96.93±2.21%），而未治疗组（28.83±10.25%）、红光组（36.63±13.08%）和STBF组（32.95±9.38%）愈合情况较差。与其他三组相比，STBF-PDT在体内显示出显著的抑菌作用。接受STBF-PDT治疗的伤口的平均活菌对数为6.80±6.98 log cfu/g（与未治疗对照相比大幅降低了99.83%）。STBF被认为是一种有利且可靠的光敏剂，其介导的光动力疗法为慢性伤口等潜在耐药性微生物感染性疾病的临床治疗提供了一种可靠且安全的方式。
