Pfab R, Schachtschabel D O, Kern H F
Strahlentherapie. 1985 Nov;161(11):711-8.
Monolayer cells of a Harding-Passey melanoma (HPM 73 cells) which were irradiated during the phase of exponential growth with an X-ray dose of 4 Gy of 8 Gy did not show any ultrastructural changes four days after 4 Gy, whereas cells irradiated with 8 Gy showed slight damages such as swollen mitochondria and vacuoles. As shown by the electron microscope, a sole addition of a sublethal quantity (6 X 10(-6) M) of quinacrine (Atebrin) or chloroquine (Resochin) did not lead to significant cell modifications. Those melanoma cells with were pre-irradiated with 8 Gy and then incubated during four days with 6 X 10(-6) M of quinacrine (Atebrin) or 6 X 10(-6) M of chloroquine (Resochin) showed severe damages. There was an increased rate of vacuoles and segregational structures in cytoplasm. The mitochondria were increased and swollen and the cellular surfaces had less microvilli. However, microtubules and microfilaments seemed more distinct. The melanin concentration increased under this treatment. The cell nuclei were increased in volume and seemed to be rather void of chromatin. These reactions of cells on quinacrine (Atebrin) and chloroquine (Resochin) are explained by the known inhibition effect exerted by these substances on DNA synthesis, especially as far as the processes of DNA reparation are concerned. The changes of the microtubule-microfilament system could be due to a correlation with the increase of digestive intracellular processes connected with the catabolism of radiation-damaged structures.
在指数生长期用4 Gy或8 Gy的X射线剂量照射的哈丁-帕西黑色素瘤单层细胞（HPM 73细胞），4 Gy照射4天后未显示任何超微结构变化，而8 Gy照射的细胞显示出轻微损伤，如线粒体肿胀和空泡形成。电子显微镜显示，单独添加亚致死量（6×10⁻⁶ M）的奎纳克林（阿的平）或氯喹（磷酸氯喹）不会导致细胞发生显著改变。那些预先用8 Gy照射，然后用6×10⁻⁶ M奎纳克林（阿的平）或6×10⁻⁶ M氯喹（磷酸氯喹）孵育4天的黑色素瘤细胞显示出严重损伤。细胞质中的空泡和分离结构的发生率增加。线粒体增多且肿胀，细胞表面的微绒毛减少。然而，微管和微丝似乎更明显。在这种处理下黑色素浓度增加。细胞核体积增大，似乎缺乏染色质。细胞对奎纳克林（阿的平）和氯喹（磷酸氯喹）的这些反应可以用这些物质对DNA合成的已知抑制作用来解释，特别是就DNA修复过程而言。微管-微丝系统的变化可能与辐射损伤结构分解代谢相关的细胞内消化过程增加有关。
