Alsagaby Reem Abdulaziz Saleh, Ansari Mohammad Azam, Alsulami Eman Mohammed, Rehman Suriya
Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Microb Pathog. 2025 Oct;207:107935. doi: 10.1016/j.micpath.2025.107935. Epub 2025 Jul 25.
Candida albicans and Candida auris are the emerging fungal pathogens having implication on hospital outbreaks and a lead cause for septicemia with increased mortality rates. The integration of green-synthesized nanoparticles with existing antifungal therapies offers a transformative approach in managing these infections. This study focuses on Phoenix dactylifera inflorescence mediated synthesis of Ag/AgO NPs for investigating their antifungal, antibiofilm, and antivirulence action. The obtained Ag/AgO NPs, 19 nm in size and mainly spherical in shape were characterized by UV-Spectroscopy, XRD, FTIR, SEM, EDX, TEM and DLS. The MIC values obtained for C. albicans and C. auris were found to be 62.5 and 125 μg/ml, respectively. Additionally, the synthesized NPs displayed significant anti-biofilm properties, reducing biofilm tested on glass tubes by 80 % at 62.5 μg/ml. The microscopic analysis showed, Ag/AgO NPs significantly inhibits the colonization and adhesions of biofilm on glass surfaces on treatment at different concentration as well as significantly inhibited the germ tube formation and disrupted the established biofilm for both Candida strains. The morphogenesis of candida from yeast to hyphae and vice-versa was also substantially inhibited at all tested concentration of Ag/AgO NPs. SEM investigation depicted, Ag/AgO NPs significantly damaged the cell membrane and cell wall that led to the loss of cell integrity that finally caused the cell death. The obtained activity of green synthesized Ag/AgO NPs, to inhibit the biofilm formation and germ tube formation as well as hyphae to yeast and yeast to hyphae transition, suggests their potential use in combination with existing antifungal drugs for the better treatment outcomes.
白色念珠菌和耳念珠菌是新出现的真菌病原体，与医院感染暴发有关，是败血症的主要病因，死亡率不断上升。将绿色合成纳米颗粒与现有的抗真菌疗法相结合，为管理这些感染提供了一种变革性方法。本研究聚焦于海枣花序介导合成Ag/AgO纳米颗粒，以研究其抗真菌、抗生物膜和抗毒力作用。通过紫外光谱、X射线衍射、傅里叶变换红外光谱、扫描电子显微镜、能谱分析、透射电子显微镜和动态光散射对获得的尺寸为19纳米且主要呈球形的Ag/AgO纳米颗粒进行了表征。发现白色念珠菌和耳念珠菌的最低抑菌浓度值分别为62.5和125μg/ml。此外，合成的纳米颗粒显示出显著的抗生物膜特性，在62.5μg/ml时可使玻璃管上测试的生物膜减少80%。显微镜分析表明，Ag/AgO纳米颗粒在不同浓度处理时可显著抑制生物膜在玻璃表面的定植和黏附，同时也显著抑制两种念珠菌菌株的芽管形成并破坏已形成的生物膜。在所有测试浓度的Ag/AgO纳米颗粒作用下，念珠菌从酵母形态向菌丝形态以及反之的形态发生也受到显著抑制。扫描电子显微镜研究表明，Ag/AgO纳米颗粒显著破坏细胞膜和细胞壁，导致细胞完整性丧失，最终导致细胞死亡。绿色合成的Ag/AgO纳米颗粒所具有的抑制生物膜形成、芽管形成以及菌丝与酵母形态转变的活性，表明它们有可能与现有的抗真菌药物联合使用以获得更好的治疗效果。
