Chen Li, Feng Yue, Hu Xiangming, Zhao Yanyun, Liu Jindi, Wu Mingyue, Li Xiao, Ha Meixuan, Qu Yanlin, Wang Qian
College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, China; State Key Laboratory of Mine Lab Disaster Prevention and Control Co-found by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, Shandong, China.
College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, China; State Key Laboratory of Mine Lab Disaster Prevention and Control Co-found by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, Shandong, China.
Environ Res. 2025 Jul 25;285(Pt 2):122446. doi: 10.1016/j.envres.2025.122446.
Carbon capture and sequestration is an effective measure to achieve China's major strategies of carbon peak and carbon neutrality, of which bio-mineralization is an important pathway. Our previous study discovered the microbial mineralization and dust suppression effect based on carbonic anhydrase (CA) in Sporosarcina pasteurii. Based on this, this study further explored the importance of Sporosarcina pasteurii in carbon sequestration by altering CO concentration. The results showed that under CO stress, Sporosarcina pasteurii enhanced CO sequestration. When the bacterium was incubated at 30 vol% CO, it enhanced the absorption of headspace CO, increasing by 49.3 %. Meanwhile, the CA activity was also enhanced by adding calcium ions. These results confirmed the mineralization potential of Sporosarcina pasteurii dependent on CO concentration. Meanwhile, different CO concentrations also have a significant effect on the distribution of calcium carbonate particle size, with higher CO concentrations resulting in smaller particles sizes. Furthermore, the transmission electron microscopy (TEM) demonstrated that high electron density particles and extracellular organic matrix secretion were visible internally in the presence of CO, indicating that extracellular polymeric substances (EPS) played an important role in CO mineralization. Besides, the hydration reaction as a mechanism for microbial-induced CO sequestration and bio-mineralization, CA accelerated CO hydration to HCO and reacted with OH and Ca to form CaCO precipitates in an alkaline environment and in the presence of a calcium source. This study provided an important basis for a deeper understanding and advancement of the microbial-mediated bio-mineralization process for efficient CO mineralization.
碳捕获与封存是实现中国碳达峰、碳中和重大战略的有效措施，其中生物矿化是一条重要途径。我们之前的研究发现了巴氏芽孢杆菌中基于碳酸酐酶（CA）的微生物矿化和抑尘作用。在此基础上，本研究通过改变CO浓度进一步探讨了巴氏芽孢杆菌在碳封存中的重要性。结果表明，在CO胁迫下，巴氏芽孢杆菌增强了碳封存能力。当该细菌在30%体积分数的CO环境中培养时，它增强了对顶空CO的吸收，增加了49.3%。同时，添加钙离子也增强了CA活性。这些结果证实了巴氏芽孢杆菌的矿化潜力依赖于CO浓度。同时，不同的CO浓度对碳酸钙粒径分布也有显著影响，较高的CO浓度导致粒径较小。此外，透射电子显微镜（TEM）显示，在有CO存在的情况下，内部可见高电子密度颗粒和细胞外有机基质分泌，这表明细胞外聚合物（EPS）在CO矿化中起重要作用。此外，水合反应作为微生物诱导的CO封存和生物矿化的一种机制，CA加速了CO水合形成HCO，并在碱性环境和有钙源存在的情况下与OH和Ca反应形成CaCO沉淀。本研究为深入理解和推进微生物介导的生物矿化过程以实现高效CO矿化提供了重要依据。
