胰蛋白酶将无义抑制因子产生的磷酸丙糖异构酶同工酶转化为类似野生型的同工酶。
Conversion by trypsin of nonsense suppressor-produced isozymes of triosephosphate isomerase to isozymes resembling the wild type.
作者信息
Baptist J N, Datta S
出版信息
J Bacteriol. 1977 Aug;131(2):682-4. doi: 10.1128/jb.131.2.682-684.1977.
Abstract
Nonsense suppressor genes caused the synthesis of new triosephosphate isomerase isozymes in Bacillus subtilis. Incubation with trypsin produced a large decrease in the apparent molecular weight of one such isozyme and simultaneously changed the electrophoretic behavior such that it resembled that of the wild-type enzyme.
摘要
无义抑制基因导致枯草芽孢杆菌中合成新的磷酸丙糖异构酶同工酶。用胰蛋白酶孵育后，其中一种同工酶的表观分子量大幅降低，同时改变了其电泳行为，使其类似于野生型酶的电泳行为。
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