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胰蛋白酶将无义抑制因子产生的磷酸丙糖异构酶同工酶转化为类似野生型的同工酶。

Conversion by trypsin of nonsense suppressor-produced isozymes of triosephosphate isomerase to isozymes resembling the wild type.

作者信息

Baptist J N, Datta S

出版信息

J Bacteriol. 1977 Aug;131(2):682-4. doi: 10.1128/jb.131.2.682-684.1977.

DOI:10.1128/jb.131.2.682-684.1977
PMID:407217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235479/
Abstract

Nonsense suppressor genes caused the synthesis of new triosephosphate isomerase isozymes in Bacillus subtilis. Incubation with trypsin produced a large decrease in the apparent molecular weight of one such isozyme and simultaneously changed the electrophoretic behavior such that it resembled that of the wild-type enzyme.

摘要

无义抑制基因导致枯草芽孢杆菌中合成新的磷酸丙糖异构酶同工酶。用胰蛋白酶孵育后,其中一种同工酶的表观分子量大幅降低,同时改变了其电泳行为,使其类似于野生型酶的电泳行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da8/235479/e33c3129e668/jbacter00303-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da8/235479/e33c3129e668/jbacter00303-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da8/235479/e33c3129e668/jbacter00303-0315-a.jpg

相似文献

1
Conversion by trypsin of nonsense suppressor-produced isozymes of triosephosphate isomerase to isozymes resembling the wild type.胰蛋白酶将无义抑制因子产生的磷酸丙糖异构酶同工酶转化为类似野生型的同工酶。
J Bacteriol. 1977 Aug;131(2):682-4. doi: 10.1128/jb.131.2.682-684.1977.
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Characterization of two new electrophoretic variants of human triosephosphate isomerase: stability, kinetic, and immunological properties.人磷酸丙糖异构酶两种新电泳变体的特性:稳定性、动力学及免疫学性质
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本文引用的文献

1
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
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The gel-filtration behaviour of proteins related to their molecular weights over a wide range.蛋白质的凝胶过滤行为与其在很宽范围内的分子量相关。
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Response of intracellular proteolysis to alteration of bacterial protein and the implications in metabolic regulation.
细胞内蛋白水解对细菌蛋白质变化的反应及其在代谢调控中的意义。
J Bacteriol. 1967 May;93(5):1527-33. doi: 10.1128/jb.93.5.1527-1533.1967.
4
The nature of the polypeptide chain termination signal.多肽链终止信号的本质。
J Mol Biol. 1971 Jun 14;58(2):513-31. doi: 10.1016/0022-2836(71)90368-8.
5
Degradation of abnormal proteins in Escherichia coli (protein breakdown-protein structure-mistranslation-amino acid analogs-puromycin).大肠杆菌中异常蛋白质的降解(蛋白质分解-蛋白质结构-错译-氨基酸类似物-嘌呤霉素)
Proc Natl Acad Sci U S A. 1972 Feb;69(2):422-6. doi: 10.1073/pnas.69.2.422.
6
Base composition of nonsense codons in Escherichia coli II. The N2 codon UAA.大肠杆菌中无义密码子的碱基组成II. N2密码子UAA。
J Mol Biol. 1967 Feb 14;23(3):391-400. doi: 10.1016/s0022-2836(67)80113-x.
7
A suppressor of nonsense mutations in Bacillus subtilis.枯草芽孢杆菌中无义突变的抑制因子。
Eur J Biochem. 1974 Aug 15;47(1):199-205. doi: 10.1111/j.1432-1033.1974.tb03683.x.
8
Pleiotropic effects of suppressor mutations in Bacillus subtilis.枯草芽孢杆菌中抑制基因突变的多效性效应。
J Bacteriol. 1974 Sep;119(3):961-75. doi: 10.1128/jb.119.3.961-975.1974.
9
Altered proteins with triosephosphate isomerase activity in suppressor-containing strains of Bacillus subtilis.枯草芽孢杆菌含抑制因子菌株中具有磷酸丙糖异构酶活性的变异蛋白。
J Bacteriol. 1974 Sep;119(3):976-85. doi: 10.1128/jb.119.3.976-985.1974.
10
Nature of the suppressor of Bacillus subtilis HA101B.枯草芽孢杆菌HA101B抑制因子的性质。
J Bacteriol. 1975 May;122(2):788-90. doi: 10.1128/jb.122.2.788-790.1975.