Ren Yuwei, Wang Feng, Sun Ruiping, Zheng Xinli, Lin Yanning, Chao Zhe
Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
Biomolecules. 2025 Jul 16;15(7):1024. doi: 10.3390/biom15071024.
Lipid metabolism is critical for the physiological activities of signal transduction, metabolic regulation, and energy provision, and Wuzhishan (WZS) pigs are a promising animal model for studying human diseases. However, lipid metabolites in the heart and liver of WZS pigs are indistinct. In this study, we detected gene expression, blood biochemical parameters, and metabolic profiles of hearts and livers of WZS and Large White (LW) pigs, and analyzed correlations between metabolites. The results showed that the fatty acid metabolic process was present in both the heart and liver, and was more dominant in the liver. Although the expression of lipid absorption-related genes of increased in the liver, levels increased in both the liver and heart; the fatty acid beta-oxidation genes and also showed increased expression. The quantity of metabolites related to lipid synthesis decreased in the liver, heart, and blood for WZS pigs compared to that of LW pigs, indicating a balance of lipid synthesis and breakdown for WZS pigs. Moreover, the lipid metabolites in the liver and heart exhibited strong correlations with each other and showed similar correlations to blood biochemical parameters, respectively. This study declared the balance of lipid metabolism in both the heart and liver, and identified their connections for WZS pigs.
脂质代谢对于信号转导、代谢调节和能量供应等生理活动至关重要，五指山（WZS）猪是研究人类疾病的一种很有前景的动物模型。然而，WZS猪心脏和肝脏中的脂质代谢物尚不明确。在本研究中，我们检测了WZS猪和大白（LW）猪心脏和肝脏的基因表达、血液生化参数及代谢谱，并分析了代谢物之间的相关性。结果表明，脂肪酸代谢过程在心脏和肝脏中均存在，且在肝脏中更为显著。虽然WZS猪肝脏中脂质吸收相关基因的表达增加，但其肝脏和心脏中的甘油三酯水平均升高；脂肪酸β氧化基因ACADM和ACOX1也表现出表达增加。与LW猪相比，WZS猪肝脏、心脏和血液中与脂质合成相关的代谢物数量减少，这表明WZS猪的脂质合成与分解处于平衡状态。此外，肝脏和心脏中的脂质代谢物相互之间表现出很强的相关性，并且分别与血液生化参数表现出相似的相关性。本研究揭示了WZS猪心脏和肝脏中脂质代谢的平衡，并确定了它们之间的联系。
