Chubakov A R, Gromova E A, Konovalov G V, Chumasov E I, Sarkisova E F
Zh Vyssh Nerv Deiat Im I P Pavlova. 1985 Sep-Oct;35(5):926-34.
The paper deals with morpho-functional development of the visual area of newborn rats cerebral cortex in conditions of prolonged (up to 40 days) organotypical cultivation and with the effect of serotonin (5-OT) on these processes. It has been shown that systematic addition of physiological concentrations of 5-OT to the nutrient medium during explantates cultivation, stimulates glia proliferation, neurones differentiation, neuropyl formation, synaptogenesis and axones myelinization. In media with 5-OT, spontaneous activity of neurones develops earlier, the number of active cells increases by 33%, neurones with periodical bursts prevail in all periods studied. Development of neurones in the medium with 5-OT changes their sensitivity to 5-OT, which becomes close to that in situ.
本文研究了新生大鼠大脑皮层视觉区在长达40天的器官型培养条件下的形态功能发育，以及血清素（5-羟色胺，5-OT）对这些过程的影响。研究表明，在培养外植体时，向营养培养基中系统添加生理浓度的5-OT，可刺激胶质细胞增殖、神经元分化、神经纤维网形成、突触发生和轴突髓鞘化。在含有5-OT的培养基中，神经元的自发活动出现得更早，活跃细胞数量增加33%，在所有研究时期，具有周期性爆发的神经元占主导。在含有5-OT的培养基中，神经元的发育改变了它们对5-OT的敏感性，使其接近原位的敏感性。
