Li Rui, Chen Xinyue, Wu Lijuan, Xie Lei, Chen Mengqiu, Qiu Yujie, Liu Fan, Chen Ji, Tian Mengliang
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China.
Curr Issues Mol Biol. 2025 Jul 11;47(7):538. doi: 10.3390/cimb47070538.
Polysaccharides isolated from Kuan (RCP) are key bioactive components with immunomodulatory, antioxidant, and anti-inflammatory effects. Their efficacy varies according to their geographic origin and processing methods. However, the systemic anti-aging mechanisms and antioxidant efficacy of RCP have not yet been comprehensively characterized. This study investigated the antioxidant and anti-aging effects of RCP in vitro and in vivo using a heat stress model, comparing rRCP (RCP from raw samples) and wRCP (RCP from wine-processed samples) from key production areas. Among these, the RCP collected from the Zhonggang region exhibited the strongest antioxidant activity. Both rRCP and wRCP enhanced worms' oxidative stress resistance, reduced their ROS levels, increased their antioxidant enzyme activities, prolonged their lifespan, and improved their reproductive capacity under thermal stress. Notably, the wRCP exhibited more pronounced benefits. Additionally, 16S rRNA sequencing revealed that RCP altered the gut microbiota's composition by increasing its microbial diversity, enriching beneficial bacteria like , and decreasing potential pathogens such as and . The treatment also led to an increased abundance of and a slight reduction in . Collectively, these findings suggest that RCP, particularly wRCP, holds promise as a therapeutic agent for combating oxidative stress and promoting longevity, in part by modulating the gut microbiome.
从宽（RCP）中分离出的多糖是具有免疫调节、抗氧化和抗炎作用的关键生物活性成分。其功效因地理来源和加工方法而异。然而，RCP的全身抗衰老机制和抗氧化功效尚未得到全面表征。本研究使用热应激模型在体外和体内研究了RCP的抗氧化和抗衰老作用，比较了来自关键产区的rRCP（生样品中的RCP）和wRCP（酒制样品中的RCP）。其中，从中港地区采集的RCP表现出最强的抗氧化活性。在热应激下，rRCP和wRCP均增强了线虫的氧化应激抗性，降低了其ROS水平，提高了其抗氧化酶活性，延长了其寿命，并改善了其繁殖能力。值得注意的是，wRCP表现出更显著的益处。此外，16S rRNA测序显示，RCP通过增加微生物多样性、富集有益细菌（如 ）和减少潜在病原体（如 和 ）来改变肠道微生物群的组成。该处理还导致 的丰度增加， 略有减少。总的来说，这些发现表明，RCP，特别是wRCP，有望作为一种治疗剂来对抗氧化应激和促进长寿，部分原因是通过调节肠道微生物群。
